{"product_id":"abcam-ab266776","title":"Abcam, ab266776, Human FANCG knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nFANCG KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 10 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 10 bp deletion in exon 3\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-FANCG, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFANCG also known as Protein FACG or Fanconi anemia group G protein is a component involved in DNA repair. It has a mass of approximately 68 kDa. This protein is mainly expressed in tissues with high proliferative rates such as bone marrow and germ cells. Mechanically FANCG plays an important role in the maintenance of genomic stability by participating in the repair processes that fix DNA interstrand crosslinks.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nFanconi anemia group G protein is an essential part of the Fanconi anemia (FA) core complex which consists of several FA proteins. This complex is indispensable for cellular resistance to DNA damage as it repairs broken DNA strands and stalls replication forks. FANCG as part of this multiprotein complex ensures proper cellular responses to genotoxic stresses and helps in preventing mutations that could be passed to daughter cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nFANCG primarily functions within the FA\/BRCA pathway an essential mechanism for DNA damage response and repair. It interacts closely with other FA proteins such as FANCA and FANCJ to facilitate the monoubiquitination of the FANCD2-FANCI complex a critical step in the DNA damage repair. Additionally the FA pathway is linked to homologous recombination repair where FANCG indirectly associates with BRCA1 and BRCA2 proteins coordinating with them to maintain genomic integrity.\u003cbr\u003e\nDefects in FANCG are directly associated with Fanconi anemia a rare genetic disorder characterized by bone marrow failure and increased cancer susceptibility. FANCG mutations disrupt its interaction within the FA complex leading to increased cellular susceptibility to DNA damage. Moreover alterations in FA pathway components including FANCG also relate to specific cancer types such as acute myeloid leukemia. The protein engages in interactions with other FA pathway proteins like FANCD2 affecting disease progress and therapeutic outcomes.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845207806121,"sku":"ab266776","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab266776","provider":"Iright","version":"1.0","type":"link"}