{"product_id":"abcam-ab267046","title":"Abcam, ab267046, Human CEACAM1 knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nCEACAM1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3 and 5 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3 and 5 bp deletion in exon 3,\u003cbr\u003e\nAntibiotic resistance:Puromycin 1µg\/mL,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-CEACAM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCEACAM1 also known as CD66a is a cell adhesion molecule that plays a critical role in various cellular processes. The protein weighs around 120 kDa and is part of the immunoglobulin superfamily. CEACAM1 is expressed on the surface of various cell types including epithelial cells leukocytes and endothelial cells. The presence of CEACAM1 is important in tissues such as the liver lungs and gastrointestinal tract where it performs essential roles in cell signaling and communication.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCEACAM1 influences cellular adhesion and immune responses by mediating cell-cell interactions. It forms homophilic dimers at the cell surface and it can participate in heterophilic interactions with other proteins making it part of complex cell signaling networks. These interactions regulate immune cell functions contributing to innate immune responses and cellular homeostasis. CEACAM1 also modulates angiogenesis endothelial cell permeability and apoptosis.\u003cbr\u003e\nPathways\u003cbr\u003e\nCEACAM1 is involved in signaling and regulatory pathways important for immune surveillance and inflammation. It is a part of the TGF-beta signaling pathway affecting cellular proliferation and differentiation. CEACAM1 interacts with proteins such as SHP-1 and SHP-2 which are essential in modulating immune responses. Additionally CEACAM1 influences the MAPK\/ERK pathway which plays a role in cell growth and survival.\u003cbr\u003e\nCEACAM1 is associated with cancer and inflammatory diseases. In colorectal cancer altered expression of CEACAM1 contributes to tumor progression and metastasis. The protein also plays a role in inflammatory bowel disease where it affects the epithelial barrier function and immune cell interaction. Through these conditions CEACAM1 interacts with other proteins like CEA and IL-6 which are pivotal in disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845065887913,"sku":"ab267046","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab267046","provider":"Iright","version":"1.0","type":"link"}