{"product_id":"abcam-ab267165","title":"Abcam, ab267165, Human DDX60 knockout A549 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nDDX60 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 13. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 13,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-DDX60, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nDDX60 also known as DExD\/H-box helicase 60 is a protein involved in antiviral responses. It has a molecular mass of approximately 180 kDa. DDX60 acts as an ATP-dependent RNA helicase unwinding RNA structures during viral replication processes. This protein is expressed mainly in immune-related tissues like the spleen and peripheral blood leukocytes indicating its role in immunity. Alternate names include DDX60L which denotes a related protein with similar functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nDDX60 plays an essential role in the detection of viral RNA acting as a sensor in the innate immune system. It forms part of a complex involved in recognizing and responding to viral infections. DDX60 interacts with other proteins like RIG-I a pivotal component in initiating antiviral signaling cascades. Through these interactions DDX60 facilitates the production of interferons which are key elements in mounting an effective immune response against viruses.\u003cbr\u003e\nPathways\u003cbr\u003e\nDDX60 functions within the RIG-I-like receptor signaling pathway an important antiviral defense mechanism. This pathway includes proteins such as MAVS and IRF3 which work together to stimulate the expression of cytokines following pathogen recognition. DDX60's activity enhances the sensitivity of this pathway boosting the host's capacity to control viral replication and spread contributing to the overall immune response.\u003cbr\u003e\nVariations in DDX60 expression have been linked to autoimmune conditions like systemic lupus erythematosus (SLE) and chronic viral infections such as hepatitis C. Abnormal regulation of this protein can lead to inappropriate immune activation or impaired response to infections. DDX60's association with RIG-I suggests its involvement in these diseases as both proteins are important in the body's effort to manage viral threats and the maintenance of immune homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845199057065,"sku":"ab267165","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab267165","provider":"Iright","version":"1.0","type":"link"}