{"product_id":"abcam-ab267298","title":"Abcam, ab267298, Human TRIO knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nTRIO KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 7. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 1 bp insertion in exon 7\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-TRIO, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTRIO also known as Triple Functional Domain Protein or Trio Rho guanine nucleotide exchange factor acts mechanically as a guanine nucleotide exchange factor. It facilitates the activation of the Rho family of small GTPases specifically Rac1 and RhoG. TRIO weighs approximately 349 kDa and is widely expressed in different tissues especially in the central nervous system. This protein modulates signal transduction associated with cytoskeletal dynamics cell migration and neuronal development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTRIO influences cellular processes like actin cytoskeleton organization and axonal outgrowth. It serves as part of multi-protein complexes that guide the intracellular signaling cascades. Particularly TRIO plays a central role in developmental pathways by promoting neuronal growth and differentiation through Rac1 activation. Its function is important for establishing the architecture of the nervous system.\u003cbr\u003e\nPathways\u003cbr\u003e\nTRIO participates in key signal transduction pathways such as the Rho GTPase cycle and the axon guidance pathway. It acts in concert with other proteins like Rac1 and RhoG to regulate cell adhesion and neuronal pathfinding. Through these pathways TRIO contributes to the polarization and directional movement of cells which is necessary for proper tissue and organ development.\u003cbr\u003e\nTRIO connects to several neurological conditions including intellectual disability and schizophrenia. Mutations in the TRIO gene as well as dysregulation of its related proteins can disrupt normal brain development and function. Particularly alterations in Rac1 and RhoG signaling have been implicated in these disorders linking TRIO to their pathogenesis and potential therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845202858153,"sku":"ab267298","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab267298","provider":"Iright","version":"1.0","type":"link"}