{"product_id":"abcam-ab267321","title":"Abcam, ab267321, Human RRAGA (Rag A) knockout HEK-293T cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nRRAGA KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 1 and 28 bp deletion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:HEK-293T,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Kidney,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, 16 bp deletion in exon 1 and 28 bp deletion in exon 1 and 2 bp deletion in exon 1\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RRAGA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRag A is a small GTPase also known as Ras-related GTP-binding protein A. It plays a role in cellular growth and proliferation. The protein operates by binding to GTP and GDP functioning as a molecular switch. Rag A's molecular weight is approximately 42 kDa. It is expressed in many tissues throughout the body indicating its widespread role in cellular processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRag A interacts with amino acids and nutrient signaling by activating the mTORC1 complex a central regulator of cell growth. It ensures correct localization of mTORC1 to the lysosomal surface facilitating its activation. The mTORC1 complex includes other proteins such as RAPTOR and mLST8 which collectively modulate cell metabolism and protein synthesis. Rag A's participation is essential for sensing nutrients and the energy state within cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nWith regards to pathways Rag A is involved in the mTOR signaling pathway and the autophagy-lysosome pathway. These two pathways are important to maintaining cellular homeostasis and responding to nutrient availability. Rag A directly interacts with Rag C another protein in this pathway to relay the presence of amino acids to mTORC1. Through these interactions Rag A aids in the regulation of cellular growth energy management and metabolism in response to environmental cues.\u003cbr\u003e\nRag A has connections to conditions like cancer and metabolic disorders. Abnormal activation of the mTOR pathway often involving Rag A can lead to uncontrolled cell proliferation seen in cancers. Additionally the protein's role in nutrient sensing links Rag A to metabolic disorders like diabetes. Alterations in Rag A activity can influence the mTORC1 pathway and misregulate associated proteins such as TSC2 contributing to disease development and progression.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845165568169,"sku":"ab267321","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab267321","provider":"Iright","version":"1.0","type":"link"}