{"product_id":"abcam-ab268947","title":"Abcam, ab268947, Recombinant human Rffl protein (Active)","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nRecombinant human Rffl protein (Active) is a Human Full Length protein, in the 1 to 363 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;70%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;70% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:GST tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:The specific activity of ab268947 was 18 nmol\/min\/mg in a ubiquitinating assay using wild-type ubiquitin protein as substrate.,\u003cbr\u003e\nAccession:Q8WZ73,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 7.5Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Rffl protein also known as RIPLET or RNF135 functions as an E3 ubiquitin ligase. It has a molecular mass of about 53 kDa. Rffl is expressed in many tissues with notable levels in cardiac and skeletal muscle as well as in the brain. The protein contributes to the ubiquitin-proteasome pathway by tagging specific substrates with ubiquitin. This tagging marks them for degradation which regulates protein levels and prevents harmful protein accumulations within cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe Rffl protein participates in the regulation of innate immune responses. It facilitates the polyubiquitination of signaling molecules involved in antiviral pathways. Rffl is not part of a large complex itself but works closely with other proteins to enhance the activation of the immune system's antiviral responses. Its activity modulates the RIG-I-like receptor (RLR) signaling pathway important for the detection of viral RNA by interacting with essential signaling molecules like RIG-I and MAVS.\u003cbr\u003e\nPathways\u003cbr\u003e\nRffl acts within the signaling cascade related to the RLR pathway and the NF-kappaB pathway. These are essential in mounting an effective immune response against viral infections. Rffl influences the pathway by modulating the function of proteins like TRAF3 and TBK1. Through these interactions it affects the downstream production of type I interferons and other immune response mediators.\u003cbr\u003e\nThe Rffl protein has been linked to immune-related conditions and viral infections. Dysregulation of Rffl activity can lead to impaired antiviral responses which contributes to increased susceptibility to viral diseases. Furthermore studies suggest connections between Rffl and proteins such as IRF3 in the progression or susceptibility to autoimmune diseases. Maintaining proper control of Rffl activity is therefore important for immune system balance and health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843958624425,"sku":"ab268947","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab268947","provider":"Iright","version":"1.0","type":"link"}