{"product_id":"abcam-ab269889","title":"Abcam, ab269889, Europium Conjugation Kit","description":"\u003cp\u003eSize: 4 x 4µg \/ 10 x 4µg \/ 1 x 40µg\u003cbr\u003e\nEuropium Conjugation Kit (ab269889) offers several standout features: - Rapid Conjugation: achieve 200 nm Europium (Eu) labeling in under 2 hours with less than 10 minutes of hands-on time. - Generates highly stable conjugates and Eu microspheres resistant to aggregation. - Eu particle Excitation (Ex): 365 nm, Emission (Em): 610 nm. - Versatility: suitable for immunoassays such as lateral flow assays.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nEuropium Conjugation Kit (ab269889) allows antibodies or proteins to be quickly and easily conjugated to high quality 200 nm Europium (Eu) chelate microspheres. The particles have been specially treated to enhance the handling of the beads and permit easy covalent attachment of antibodies and other proteins.\u003cbr\u003e\nThe Eu microspheres in this kit are freeze dried. The conjugation reaction is initiated simply by reconstituting the dry mixture with the antibody, which becomes attached (via lysine residues) to the specially treated surface.\u003cbr\u003e\nIt takes 30 seconds to set up the conjugation, the hands-on time for the conjugation procedure is about 3 minutes and the conjugate is ready to use within 35 minutes. The biomolecule is simply pipetted into a vial containing the particles, the conjugation is allowed to occur and then a centrifugation allows a buffer exchange.\u003cbr\u003e\nThe surface treatment makes the particles resistant to aggregation and the Eu fluorescence signal allows a higher sensitivity to be reached in immunoassays such as lateral flow assays.\u003cbr\u003e\nAdditionally, unlike passive methods the conjugation procedure has only a weak dependence on the isoelectric point of the antibody. Consequently, extensive trials at different pH values are not required; all antibodies can be conjugated at one of two pHs, both of which are supplied in this kit (Reaction Buffers A and B).\u003cbr\u003e\nBenefits of Europium Conjugation Kit:\u003cbr\u003e\nEasy and rapid conjugation - Conjugates ready to use in 35 minutes\u003cbr\u003e\n15-fold higher sensitivity compared with other common particles\u003cbr\u003e\nGeneration of stable conjugates\u003cbr\u003e\nResistant to aggregation\u003cbr\u003e\nNo harsh resuspension methods required\u003cbr\u003e\nFully scalable- Easy transfer from R\u0026amp;D to manufacturing\u003cbr\u003e\nBuffer requirements:\u003cbr\u003e\nWe recommend that the antibody is at a concentration of 1 mg\/mL in 10-50 mM MES, HEPES or MOPS at pH 6-7 (with no other components e.g. salt or azide) prior to conjugation. For incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits for Nanoparticles. To learn more about incompatible buffers, please refer to the protocol booklet.\u003cbr\u003e\nOther Products\u003cbr\u003e\nLearn about our Lightning-Link® antibody labeling kits and their advantages\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company. It was previously called 200nm Europium Conjugation Kit. Expedeon product code 1200-0040 (4 reaction mini kit) is the same as the 4 x 4 µg size of this product, code 1200-0100 (10 reaction mini kit) is the same as the 10 x 4 µg size, and code 1200-0120 (1 reaction midi kit) is the same as the 1 x 40 µg size of this product.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847601705129,"sku":"ab269889","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab269889","provider":"Iright","version":"1.0","type":"link"}