{"product_id":"abcam-ab269898","title":"Abcam, ab269898, Atto 633 Conjugation Kit (Fast) - Lightning-Link®","description":"\u003cp\u003eSize: 100µg \/ 3 x 10µg \/ 3 x 100µg \/ 1mg\u003cbr\u003e\nAtto 633 Conjugation Kit (Fast) - Lightning-Link ®  (ab269898) offers several standout features: - Rapid Conjugation: achieve Atto 633 labeling in under 2 hours with just 30 seconds of hands-on time. - High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies. - Versatility: suitable for conjugating antibodies, proteins, and peptides. Atto 633-labeled antibodies can be used immediately in applications such as Western blot, Flow cytometry, ELISA, and Immunohistochemistry (IHC) without further purification.\u003cbr\u003e\nProduct details:\u003cbr\u003e\nAtto 633 Conjugation Kit (Fast) - Lightning-Link\u003cbr\u003e\n(ab269898) provides an easy-to-use, one step procedure that allows researchers to covalently label proteins, peptides and other biomolecules containing primary amines with Atto 633 with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.\u003cbr\u003e\nThe antibody to be labeled should be purified, in an appropriate buffer for conjugation and at a suitable concentration.\u003cbr\u003e\nThe excitation and emmision wavelengths for Atto 633 are Ex: 630nm, Em: 651nm.\u003cbr\u003e\nLearn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid\u003cbr\u003e\nantibody purification and concentration kits\u003cbr\u003e\n. Use the\u003cbr\u003e\nto learn more about the technology, or about conjugating other proteins and peptides to Atto 633.\u003cbr\u003e\nCustom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link\u003cbr\u003e\nRapid Atto 633 Labeling Kit. 353-0015 is the same as the 1 mg size. 353-0010 is the same as the 3 x 100 ug size. 353-0030 is the same as the 3 x 10 ug size. 353-0005 is the same as the 100 μg size.\u003cbr\u003e\nAmount and volume of antibody for conjugation to Atto 633\u003cbr\u003e\nKit size\u003cbr\u003e\nRecommended\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum\u003cbr\u003e\namount of antibody\u003cbr\u003e\nMaximum antibody\u003cbr\u003e\nvolume\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 10 μg\u003cbr\u003e\n3 x 20 μg\u003cbr\u003e\n3 x 10 μL\u003cbr\u003e\n100 μg\u003cbr\u003e\n1 x 100 μg\u003cbr\u003e\n1 x 200 μg\u003cbr\u003e\n1 x 100 μL\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 100 μg\u003cbr\u003e\n3 x 200 μg\u003cbr\u003e\n3 x 100 μL\u003cbr\u003e\n1 x 1 mg\u003cbr\u003e\n1 x 2 mg\u003cbr\u003e\n1 x 1 mL\u003cbr\u003e\nUsing the maximum amount of antibody may result in less labeling per antibody.\u003cbr\u003e\nIdeal antibody concentration is 1mg\/ml. 0.5 - 1 mg\/ml can be used if the maximum antibody volume is not exceeded. Antibodies \u0026gt; 2mg\/ml or \u0026lt; 0.5 mg\/ml should be diluted \/concentrated.\u003cbr\u003e\nBuffer Requirements for Conjugation\u003cbr\u003e\nBuffer should be pH 6.5-8.5.\u003cbr\u003e\nCompatible buffer constituents\u003cbr\u003e\nIf a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.\u003cbr\u003e\n50mM \/ 0.6% Tris\u003cbr\u003e\n0.1% BSA\u003cbr\u003e\n50% glycerol\u003cbr\u003e\n0.1% sodium azide\u003cbr\u003e\nPotassium phosphate\u003cbr\u003e\nSodium chloride\u003cbr\u003e\nSucrose\u003cbr\u003e\nSodium citrate\u003cbr\u003e\nTrehalose\u003cbr\u003e\nTris buffered saline is almost always ≤ 50 mM \/ 0.6%\u003cbr\u003e\nBSA can also interfere with the use of the conjugated antibody in tissue staining.\u003cbr\u003e\nIncompatible buffer constituents\u003cbr\u003e\nThiomerosal\u003cbr\u003e\nProclin\u003cbr\u003e\nGlycine\u003cbr\u003e\nArginine\u003cbr\u003e\nGlutathione\u003cbr\u003e\nIf a constituent of the buffer containing your antibody or protein is not listed above, please contact us.\u003cbr\u003e\nOnly purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.\u003cbr\u003e\nStoring and handling conjugation kits\u003cbr\u003e\nLyophilized Lightning-Link\u003cbr\u003e\ncomponents are hygroscopic.\u003cbr\u003e\nKits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847601017001,"sku":"ab269898","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab269898","provider":"Iright","version":"1.0","type":"link"}