{"product_id":"abcam-ab270018","title":"Abcam, ab270018, Cobalt NTA Affinity Resin - Amintra","description":"\u003cp\u003eSize: 10mL \/ 25mL \/ 100mL\u003cbr\u003e\nCobalt NTA Affinity Resin - Amintra (ab270018) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions.\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 6 - 8Constituents: Agarose beads, 20% Ethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCobalt NTA Affinity Resin - Amintra (ab270018) is designed for simple, rapid His-tagged recombinant protein purification from a cell lysate under native or denaturing conditions. Metal chelate affinity chromatography is a rapid one step purification, which removes most contaminants and can achieve purities close to homogeneity. The rapid purification protocols provided in the protocol book along with the NTA resin for affinity chromatography permit the recovery of high levels of pure recombinant protein in minutes. Large numbers of samples can be processed at the same time. Recombinant proteins purified using Cobalt NTA Affinity Resin - Amintra (ab270018) may be used in a wide range of structure and activity based laboratory procedures.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Cobalt NTA Affinity Resin - Amintra. Expedeon product codes ACO0010 (10 mL Medium), ACO0025 (25 mL Medium) and ACO0100 (100 mL Medium) are the same as the current sizes: 10 mL, 25 mL, and 100 mL, respectively.\u003cbr\u003e\nCharacterization of the resin\u003cbr\u003e\nSupporting matrix:\u003cbr\u003e\nHighly cross-linked 6% agarose\u003cbr\u003e\nCharged metal ion:\u003cbr\u003e\nBead size range:\u003cbr\u003e\n45-165 μm\u003cbr\u003e\nRecommended working pH Short term:\u003cbr\u003e\npH 2.0-14.0 – Long term: pH 3.0-12.0\u003cbr\u003e\nTypical binding capacity:\u003cbr\u003e\n\u0026gt; 20 mg 6x HIS-tagged recombinant protein\/ml resin\u003cbr\u003e\nMaximum pressure:\u003cbr\u003e\n0.3MPa (3 bar)\u003cbr\u003e\nChemical stability:\u003cbr\u003e\nSolubility in water:\u003cbr\u003e\nInsoluble\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847578276009,"sku":"ab270018","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab270018","provider":"Iright","version":"1.0","type":"link"}