{"product_id":"abcam-ab270049","title":"Abcam, ab270049, BaseMuncher Endonuclease (TurboNuclease)","description":"\u003cp\u003eSize: 25000Unit\u003cbr\u003e\nBaseMuncher Endonuclease (ab270049) is a non-specific endonuclease that is used for the removal of nucleic acid from protein samples. - Hydrolyzes both single-stranded and double-stranded, circular and linear nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1-4 bases in length - Free of protease activity - Can replace crude Dnase1 in several applications\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEndonuclease enzyme BaseMuncher is a non-specific endonuclease that is used for the removal of nucleic acid from protein samples. Our ultrapure endonuclease hydrolyzes both single stranded and double stranded, along with circular and linear nucleic acids (DNA and RNA) to 5-phosphorylated oligonucleotides of 1-4 bases in length.\u003cbr\u003e\nRecombinantly produced in E. coli using the Benzonase gene in a proprietary process, BaseMuncher is a highly purified homodimer of 27kDa subunits that have exceptionally high specific activity and is completely free of protease activity. BaseMuncher is ideal for reducing viscosity during protein purification and sample preparation.\u003cbr\u003e\nOne Unit of BaseMuncher converts 1.0 OD\u003cbr\u003e\nof salmon sperm DNA into acid soluble nucleotides in 30 minutes at 37°C in a reaction buffer of 50mM Tris-HCl, pH 8.0 and 1mM MgCl\u003cbr\u003e\n. This corresponds to complete digestion of 50μg of salmon sperm DNA into oligonucleotides.\u003cbr\u003e\nFeatures \u0026amp; Benefits:\u003cbr\u003e\nExceptionally high endonuclease specificity for DNA and RNA\u003cbr\u003e\nUltrapure to \u0026gt;99%\u003cbr\u003e\nFree of protease activity\u003cbr\u003e\nHigh purity and high concentration grade\u003cbr\u003e\nEasy protein purification –Reduced viscosity during sample preparation\u003cbr\u003e\n100x greater activity than DNAase\u003cbr\u003e\nCheaper than Benzonase; but the same quality and efficiency.\u003cbr\u003e\nGeneral notes:\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company. Expedeon product code BM0025 was previously called BaseMuncher Endonuclease \u0026gt;99% Ultrapure - 25,000 Units (1 x 100 μL) and is the same as the 25,000 unit size of this product. Expedeon product code BM0100 was previously called BaseMuncher Endonuclease \u0026gt;99% Ultrapure - 100,000 Units (4 x 100 μL) and is is the same as the 100,000 unit size.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847574048937,"sku":"ab270049","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab270049","provider":"Iright","version":"1.0","type":"link"}