{"product_id":"abcam-ab270308","title":"Abcam, ab270308, Protein A Resin - Amintra","description":"\u003cp\u003eSize: 5mL \/ 25mL \/ 200mL\u003cbr\u003e\nProtein A Resin - Amintra® (ab270308) is is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media.\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 6 - 8Constituents: 50% Agarose beads, 30% Deionized Water (H20), 20% Ethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProtein A Resin - Amintra\u003cbr\u003e\n(ab270308) is is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and cell culture media. The recombinant protein A ligand is coupled to highly cross-linked 4% agarose resin. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin - Amintra\u003cbr\u003e\nis greater than 40 mg human IgG per mL settled resin. The dynamic binding capacity varies depending on several factors such as the target antibody, flow rate during binding, etc.\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Amintra® Protein A Resin. Expedeon product codes APA0005 (5 mL Medium), APA0025 (25 mL Medium) and APA0100 (100 mL Medium) are the same as the current sizes: 5 mL, 25 mL, and 100 mL, respectively.\u003cbr\u003e\nCharacterization of the Resin:\u003cbr\u003e\nSupporting matrix:\u003cbr\u003e\nHighly cross-linked 4% agarose supplied as 50% slurry\u003cbr\u003e\nLigand:\u003cbr\u003e\nRecombinant Protein A\u003cbr\u003e\nBead size range:\u003cbr\u003e\n45-165 μm\u003cbr\u003e\nRecommended working pH:\u003cbr\u003e\n2.5-9.0\u003cbr\u003e\nTypical binding capacity:\u003cbr\u003e\n\u0026gt;40 mg Human IgG \/ml resin\u003cbr\u003e\nMaximum Flow rate:\u003cbr\u003e\nUp To 300 cm\/h\u003cbr\u003e\nMaximum pressure:\u003cbr\u003e\n0.3MPa (3 bar)\u003cbr\u003e\nChemical stability:\u003cbr\u003e\nHigh – Stable in all aqueous buffers commonly used in Protein A chromatography:\u003cbr\u003e\n10 mM HCl (pH 2)\u003cbr\u003e\n1 mM NaOH (pH 11)\u003cbr\u003e\n0.1 M sodium citrate\/HCl (pH 3)\u003cbr\u003e\n6 M guanidine-HCl\u003cbr\u003e\n20% ethanol\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n1x PBS containing 20% ethanol\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847581257897,"sku":"ab270308","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab270308","provider":"Iright","version":"1.0","type":"link"}