{"product_id":"abcam-ab270405","title":"Abcam, ab270405, Anti-PELP1 antibody [EPR22863-99] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal PELP1 antibody. Carrier free. Suitable for WB, ICC\/IF, Flow Cyt (Intra) and reacts with Human samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR22863-99,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nApplications:ICC\/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nab270405 is the carrier-free version of\u003cbr\u003e\nab270398\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Proline Glutamic acid and Leucine-rich Protein 1 (PELP1) also known as Modulator of Nongenomic Activity of Estrogen Receptor (MNAR) plays a significant role in chromatin remodeling and transcriptional regulation. PELP1 has a molecular mass of approximately 160 kDa. It is broadly expressed in various tissues with particularly high levels in reproductive tissues and the brain. PELP1 participates in the recruitment of histone-modifying enzymes and acts as a scaffolding protein that interacts with several nuclear receptors and transcription factors.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPELP1 acts within the nucleus and is central to gene expression regulation. It is an integral part of diverse protein complexes that engage in remodeling chromatin and modifying transcriptional activity. PELP1 interacts with estrogen receptors where it modulates both genomic and non-genomic signaling pathways affecting gene expression programs related to cell growth and differentiation. Additionally PELP1 associates with various other hormone receptors and transcription factors assisting in the intricate regulation of cell functions.\u003cbr\u003e\nPathways\u003cbr\u003e\nCellular growth and differentiation rely on the signaling roles that PELP1 performs. PELP1 is involved in the estrogen signaling pathway where it closely interacts with estrogen receptor alpha (ERα). This interaction links estrogen signaling to the cell cycle machinery and PI3K\/AKT pathway influencing cell proliferation and survival. Another pathway in which PELP1 plays a critical role is the MAPK\/ERK pathway where it cooperates with Src kinase highlighting its importance in cell signaling networks that regulate growth and differentiation.\u003cbr\u003e\nPELP1 holds a strong association with hormone-driven cancers particularly breast and prostate cancers. In these contexts PELP1's interaction with estrogen and androgen receptors can dramatically alter tumor progression suggesting its role as an oncogene. Additionally aberrant expression and activity of PELP1 have implications in neurological disorders. In breast cancer PELP1 collaborates with ERα to drive estrogen-mediated growth whereas in prostate cancer it engages with androgen receptor (AR) promoting cell proliferation and resistance to therapy.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851865739433,"sku":"ab270405","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab270405","provider":"Iright","version":"1.0","type":"link"}