{"product_id":"abcam-ab270538","title":"Abcam, ab270538, Maltose Binding Protein Affinity Resin - Amintra","description":"\u003cp\u003eSize: 10mL \/ 100mL\u003cbr\u003e\nProduct overviewMBP tagging of recombinant proteins can result in increased expression levels and higher solubility of the target protein, while allowing high purity recovery.\u003cbr\u003e\nKey facts\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nStorage buffer:pH: 6 - 8Constituents: Agarose beads, 20% Ethanol\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nProduct overview\u003cbr\u003e\nMBP tagging of recombinant proteins can result in increased expression levels and higher solubility of the target protein, while allowing high purity recovery. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Due to the increased solubility, the tag can be extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).\u003cbr\u003e\nMaltose Binding Protein Affinity Resin - Amintra (ab270538) is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). The base matrix of this resin is a robust, highly cross-linked 6% agarose, optimized to give high physical and chemical stability and high binding specificity. The evenly sized beads ensure that MBP-tagged proteins elute in narrow peaks, minimising the need for further time-consuming concentration steps.\u003cbr\u003e\nAffinity purification using Maltose Binding Protein Affinity Resin - Amintra takes place under physiological conditions, and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.\u003cbr\u003e\nThe characteristics of the resin:\u003cbr\u003e\nMatrix\u003cbr\u003e\nHighly crosslinked 6% agarose\u003cbr\u003e\nLigand\u003cbr\u003e\nDextrin\u003cbr\u003e\nCapacity\u003cbr\u003e\n\u0026gt;10 mg MBP tagged protein (80 kDa)\/mL medium\u003cbr\u003e\nParticle Size\u003cbr\u003e\n45-165 μm\u003cbr\u003e\nMaximum Pressure\u003cbr\u003e\n0.3 MPa, 3 bar\u003cbr\u003e\npH Stability\u003cbr\u003e\nStorage Buffer\u003cbr\u003e\n20% ethanol\u003cbr\u003e\nGeneral notes\u003cbr\u003e\nThis product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Maltose Binding Protein (MBP) Affinity Resin. Expedeon product code AMB0015 (15 mL Medium) and AMB00100 (100 mL of Medium) are the same as the current sizes: 15 mL and 100 mL, respectively.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847575818409,"sku":"ab270538","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab270538","provider":"Iright","version":"1.0","type":"link"}