{"product_id":"abcam-ab270883","title":"Abcam, ab270883, Human IL-2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests \/ 1 x 384Tests \/ 10 x 96Tests\u003cbr\u003e\nHuman IL-2 ELISA Kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human interleukin-2 (IL-2) with a sensitivity of 32.1 pg\/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step. - Colorimetric Sandwich ELISA - 450 nm readout; compatible with any standard plate reader - Available in different formats to meet various throughput needs, including 384-well plates for higher throughput - Broad sample compatibility – suitable for cell culture media, cell culture supernatant, serum and plasma - Cited in over 10 publications\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture media, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 32.1 pg\/mL,\u003cbr\u003e\nRange:39 - 2500 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman IL-2 ELISA Kit ab270883 is a rapid single-wash 90-min ELISA kit to measure Human IL-2 in cell culture media, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 32.1 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman IL-2 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nThis kit recognizes both native and recombinant human IL-2 protein in stimulated serum, plasmas, and cell culture supernatant. Urine, milk, saliva, and cell and tissue extract samples have not been tested with this kit. Recombinant human IL-2R alpha was prepared at 5,000 pg\/mL and assayed for cross reactivity. No cross-reactivity was observed. Recombinant human IL-2R alpha was prepared at 5,000 pg\/mL and tested for interference. No interference with was observed.\u003cbr\u003e\nHuman IL-2 ELISA Kit ab270883 protocol summary:\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450nm\u003cbr\u003e\nHow other researchers are using Human IL-2 ELISA Kit ab270883\u003cbr\u003e\nHuman IL-2 ELISA Kit ab270883 has been used to study the role of long noncoding RNA in immunotherapy resistance\u003cbr\u003e\nand exosomal effects of HLA transfer on T lymphocytes in Celiac Disease\u003cbr\u003e\nReferences:\u003cbr\u003e\nHui-Yan Luo et al. 2023, PMID: 37712124,\u003cbr\u003e\nValerie Brid O'Leary et al. 2022, PMID: 35682780\u003cbr\u003e\nSave your precious samples\u003cbr\u003e\nOur 384-well Human IL-2 ELISA Kit only requires a maxmum of 12.5µL\u003cbr\u003e\nRelated and recommended products\u003cbr\u003e\nHuman IL-2 ELISA Kit ab270883 is commonly used to measure human interleukin 2 as a marker of inflammation.\u003cbr\u003e\nOther ELISA kits to measure inflammatory markers include:\u003cbr\u003e\n- Human TNF alpha ELISA kit\u003cbr\u003e\nab181421\u003cbr\u003e\n- Human IL-6 ELISA kit\u003cbr\u003e\nab178013\u003cbr\u003e\n- Human IFN gamma ELISA kit\u003cbr\u003e\nab174443\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843625701545,"sku":"ab270883","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab270883","provider":"Iright","version":"1.0","type":"link"}