{"product_id":"abcam-ab271721","title":"Abcam, ab271721, Recombinant human PRMT5 + MEP50 protein (Active)","description":"\u003cp\u003eSize: 20µg\u003cbr\u003e\nRecombinant human PRMT5 + MEP50 protein (Active) is a Human Full Length protein, in the 2 to 637 aa range, expressed in Baculovirus infected Sf9 cells, with \u0026gt;89%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;89% SDS-PAGE,\u003cbr\u003e\nExpression system:Baculovirus infected Sf9 cells,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Assay Conditions: 50 μl reaction mix (20 mM phosphate buffer pH 7.4, 20 μM S-adenosyl methionine, and 10-100 ng methyltransferase PRMT5) add to the wells coated with the substrate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.,\u003cbr\u003e\nAccession:O14744,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nStorage buffer:pH: 8Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPRMT5 also known as Protein Arginine Methyltransferase 5 and its cofactors like MEP50 (also called WDR77) regulate arginine methylation on histones and other proteins. PRMT5 is an enzyme weighing approximately 72 kDa and is expressed in various tissues including the brain heart and kidneys. MEP50 however acts as an important partner in forming the PRMT5-MEP50 complex enhancing its enzymatic activity. Together they play a significant role in symmetric dimethylation of arginine residues impacting gene expression regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe PRMT5 and MEP50 complex contributes to numerous cellular processes. The complex influences cellular processes by altering histone modifications which can affect chromatin structure and gene transcription. By methylating non-histone proteins it participates in RNA processing signal transduction and protein interactions. The activity of PRMT5-MEP50 is vital for maintaining cellular differentiation proliferation and apoptosis showing importance in normal developmental processes and stem cell maintenance.\u003cbr\u003e\nPathways\u003cbr\u003e\nPRMT5 and MEP50 are involved in several key signaling pathways including the Wnt signaling and cell cycle regulation pathways. Within these PRMT5 collaborates with proteins like Cyclin D1 which aids in cell cycle progression. The enzyme also associates with proteins in the PI3K\/AKT signaling pathway. These partnerships enable PRMT5 and MEP50 to influence important aspects of cell fate and functionality integrating signals that govern growth and division.\u003cbr\u003e\nAberrant PRMT5-MEP50 activity links to various cancers such as lung and breast cancer. Overexpression of PRMT5 contributes to tumorigenesis by promoting uncontrolled cell proliferation and survival. Furthermore altered methylation patterns driven by PRMT5 are involved in neurodegenerative disorders like amyotrophic lateral sclerosis (ALS). Through these conditions PRMT5 often works in tandem with proteins like p53 which regulates cell cycle and apoptosis further demonstrating the role of PRMT5 and MEP50 in disease progression and potential as therapeutic targets.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843949482153,"sku":"ab271721","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab271721","provider":"Iright","version":"1.0","type":"link"}