{"product_id":"abcam-ab273297","title":"Abcam, ab273297, Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric)","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nAngiotensin II Converting Enzyme (ACE2) Activity Assay Kit ab273373 uses the ability of an active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Fluorescent,\u003cbr\u003e\nSample types:Tissue, Suspension cells, Adherent cells,\u003cbr\u003e\nAssay type:Enzyme activity (quantitative),\u003cbr\u003e\nSensitivity:= 400 µU,\u003cbr\u003e\nAssay Platform:Microplate\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAngiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric) (\u003cbr\u003e\nab273373\u003cbr\u003e\n) uses the ability of active ACE2 to cleave a synthetic MCA based peptide substrate to release a free fluorophore. The released MCA can be easily quantified using a fluorescence microplate reader.\u003cbr\u003e\nThe kit also includes an ACE2 specific inhibitor that can differentiate the ACE2 activity from other proteolytic activity. The kit can detect as low as 0.4 mU. The assay kit is simple and can be used in a high-throughput format.\u003cbr\u003e\nA negative control should be run for each sample to differentiate ACE2 activity from other proteolytic activity in your sample\u003cbr\u003e\nOther Notes\u003cbr\u003e\nThis product was previously called K897 Biovision Angiotensin II Converting Enzyme (ACE2) Activity Assay Kit (Fluorometric). Biovision was acquired by Abcam in 2021.\u003cbr\u003e\nThe Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ACE2 protein also known as angiotensin-converting enzyme 2 is an essential component in the renin-angiotensin system. It functions mechanically by converting the hormone angiotensin II to angiotensin-(1-7) which helps regulate blood pressure and fluid balance. The molecular weight of ACE2 is approximately 120 kDa. This protein is expressed in various tissues particularly the lungs heart kidneys and gastrointestinal tract. In cultured cells like Caco-2 cells researchers often study this expression.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe ACE2 protein plays an important role in the regulation of cardiovascular and renal functions. It is a single-pass type I membrane protein and its activity reduces inflammation and oxidative stress in cells. ACE2 does not function as part of a larger protein complex but its enzymatic conversion has a substantial impact on reducing the effects of angiotensin II in the body leading to vasodilation and decreased blood pressure.\u003cbr\u003e\nPathways\u003cbr\u003e\nACE2 involvement is significant in the renin-angiotensin system and the kallikrein-kinin system. These pathways are essential for maintaining cardiovascular homeostasis. In the renin-angiotensin system ACE2 works in opposition to angiotensin-converting enzyme (ACE) balancing the effects through the production of angiotensin-(1-7) from angiotensin II. Additionally ACE2 interacts indirectly with proteins like angiotensin receptor type 1 (AT1) and angiotensin receptor type 2 (AT2) ensuring proper signaling and physiological responses.\u003cbr\u003e\nACE2 links closely with conditions such as hypertension and COVID-19. Increased activity of angiotensin II due to low ACE2 levels contributes to hypertension. In infectious disease SARS-CoV-2 virus responsible for COVID-19 uses ACE2 as an entry receptor to initiate infection in host cells. This interaction highlights the importance of ACE2 in disease pathogenesis and has prompted interest in ACE2 as a potential therapeutic target.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843560198313,"sku":"ab273297","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab273297","provider":"Iright","version":"1.0","type":"link"}