{"product_id":"abcam-ab275521","title":"Abcam, ab275521, Human IFNG knockout Jurkat cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nIFNG KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR\/Cas9, Homozygous: 92bp deletion and 5bp insertion in exon 3 \u0026amp; intron 3-4.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:Jurkat,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Blood,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,Western blot,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, Homozygous: 92bp deletion and 5bp insertion in exon 3 \u0026amp; intron 3-4,\u003cbr\u003e\nDisease:Non-Hodgkin Lymphoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nTreatments:\u003cbr\u003e\nHuman IFNG knockout Jurkat cell lysate - BFA (5 μg\/ml, last 5h)\u003cbr\u003e\nHuman wild-type Jurkat cell lysate - BFA (5 μg\/ml, last 5h)\u003cbr\u003e\nHuman IFNG knockout Jurkat cell lysate - Ionomycin (1 μg\/ml, 6h), PMA (25 ng\/ml, 6h) and BFA (5 μg\/ml, last 5h)\u003cbr\u003e\nHuman wild-type Jurkat cell lysate - Ionomycin (1 μg\/ml, 6h), PMA (25 ng\/ml, 6h) and BFA (5 μg\/ml, last 5h)\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-IFNG, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInterferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.\u003cbr\u003e\nPathways\u003cbr\u003e\nIFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.\u003cbr\u003e\nIFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845126869161,"sku":"ab275521","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab275521","provider":"Iright","version":"1.0","type":"link"}