{"product_id":"abcam-ab277422","title":"Abcam, ab277422, Human ERAP2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman ERAP2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ERAP2 in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 1.23 ng\/mL,\u003cbr\u003e\nRange:1.229 - 300 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman ERAP2 ELISA Kit (ab277422) is an\u003cbr\u003e\nin-vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human ERAP2 in serum, plasma and cell culture supernatants\u003cbr\u003e\nThis assay employs an antibody specific for Human ERAP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ERAP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ERAP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ERAP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ERAP2 protein also known as endoplasmic reticulum aminopeptidase 2 plays an important mechanical role in the cell. It belongs to the M1 zinc metallopeptidase family and has a molecular weight of about 110 kDa. ERAP2 is widely expressed in a range of tissues with notable expression in immune-related organs such as the spleen and lymph nodes. It functions to trim peptides in the endoplasmic reticulum preparing them for presentation by major histocompatibility complex (MHC) class I molecules.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe trimming activity of ERAP2 assists the immune system in recognizing antigens. It works closely with other components of the antigen processing machinery including the proteasome and transporter associated with antigen processing (TAP). ERAP2 can form a functional complex with ERAP1 another aminopeptidase enhancing the efficiency of peptide processing. The interworking of these proteins is vital for maintaining the diversity and specificity of peptides generated for the immune defense.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe ERAP2 protein is involved in the antigen processing and presentation pathway a critical component of the body's adaptive immune response. This pathway ensures foreign or aberrant peptides are presented to CD8+ T cells triggering an immune response. Related proteins such as ERAP1 and TAP collaborate with ERAP2 within this pathway highlighting an interconnected mechanism for immune surveillance and activity.\u003cbr\u003e\nERAP2 has associations with autoimmune diseases and inflammatory disorders. Variants and dysregulation of ERAP2 expression have links to ankylosing spondylitis a type of arthritis causing inflammation in the spine's joints and ligaments. Similarly altered ERAP2 function is implicated in inflammatory bowel disease. The interplay with ERAP1 is significant here as both proteins contribute to the altered antigen processing observed in these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855660961961,"sku":"ab277422","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab277422","provider":"Iright","version":"1.0","type":"link"}