{"product_id":"abcam-ab277447","title":"Abcam, ab277447, Human Galectin 10 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Galectin 10 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Galectin 10 in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.45 ng\/mL,\u003cbr\u003e\nRange:0.41 - 100 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Galectin 10 ELISA Kit (ab277447) is an\u003cbr\u003e\nin-vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 10 in serum (human Galectin 10 concentration is low in normal serum\/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human Galectin 10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Galectin 10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Galectin 10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Galectin 10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGalectin-10 also known as Charcot-Leyden crystal protein is part of the galectin family of proteins. It functions by binding to carbohydrates due to its unique lectin properties. With a molecular mass of approximately 16.5 kDa this protein is mainly expressed in eosinophils and basophils cells that are significant in the immune system. Galectin-10 is secreted by these cells and also stored in their granules suggesting its importance in immune responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGalectin-10 influences cellular processes like apoptosis cell adhesion and immune cell migration contributing to immune regulation. It is not well known to form part of large complexes but its release has implications on cell signaling and tissue remodeling. The presence of galectin-10 in eosinophils suggests a role in inflammation given their involvement in allergic responses and asthma.\u003cbr\u003e\nPathways\u003cbr\u003e\nStudies associate galectin-10 with the allergy response and eosinophil apoptosis pathways. This protein cooperates with other galectin family members like galectin-3 in modulating cellular responses. Signaling interactions of galectin-10 impact pathways involving cell survival and death highlighting its role in immune system dynamics.\u003cbr\u003e\nResearchers link galectin-10 to conditions such as asthma and eosinophilic disorders. Eosinophil activity in these diseases often correlates with elevated galectin-10 levels in tissues and bloodstream. Connections exist between galectin-10 and other inflammation-related proteins like interleukins influencing disease progression and severity through their regulatory roles in immune responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855653916841,"sku":"ab277447","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab277447","provider":"Iright","version":"1.0","type":"link"}