{"product_id":"abcam-ab277466","title":"Abcam, ab277466, Human ARSB ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman ARSB ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ARSB in Human in Biofluids, Cell Culture Media samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.32 ng\/mL,\u003cbr\u003e\nRange:0.307 - 75 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman ARSB ELISA Kit (ab277466) is an\u003cbr\u003e\nin-vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of Human ARSB in serum, plasma and cell culture supernatants.\u003cbr\u003e\nThis assay employs an antibody specific for Human ARSB coated on a 96-well plate. Standards and samples are pipetted into the wells and Human ARSB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human ARSB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human ARSB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe ARSB protein also known as arylsulfatase B or N-acetylgalactosamine-4-sulfatase is an enzyme weighing approximately 62 kDa. It plays a role in breaking down glycosaminoglycans in the lysosomes. ARSB is highly expressed in tissues such as the liver kidney and skin where its enzymatic activity is important for normal cellular metabolism.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nARSB functions in the degradation of dermatan sulfate and chondroitin sulfate types of glycosaminoglycans. It works as a monomer and is not part of a larger protein complex. This enzyme targets specific sulfated molecules facilitating their breakdown and recycling within the lysosomes.\u003cbr\u003e\nPathways\u003cbr\u003e\nARSB is actively involved in the lysosomal degradation pathway one of the key cellular processes for waste removal and recycling of macromolecules. This pathway includes collaboration with other lysosomal enzymes such as iduronate-2-sulfatase to complete the breakdown of complex molecules. ARSB's function in coordination with these enzymes supports cellular maintenance and metabolic balance.\u003cbr\u003e\nARSB malfunction is directly linked to the lysosomal storage disorder Mucopolysaccharidosis VI (Maroteaux-Lamy syndrome) characterized by the accumulation of glycosaminoglycans. This deficiency can lead to several symptoms including skeletal abnormalities and organomegaly. ARSB has also shown relevance in some inflammatory disorders where alterations in glycosaminoglycan breakdown impact cellular communication and tissue homeostasis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855651033257,"sku":"ab277466","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab277466","provider":"Iright","version":"1.0","type":"link"}