{"product_id":"abcam-ab277514","title":"Abcam, ab277514, Anti-SARS-CoV-2 Spike Ectodomain antibody [CV1] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nHuman Recombinant Monoclonal SPIKE antibody. Carrier free. Suitable for I-ELISA and reacts with Recombinant full length protein - SARS-CoV-2 samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Human,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:CV1,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nLight chain type:lambda,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nApplications:I-ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nCV1 antibody cross-reacts with SARS-CoV-2 Spike Ectodomain (including S1 domain), but not with SARS-CoV-2 Spike RBD. CV1 does not demonstrate significant neutralising ability in in-direct ELISA, measuring competitive binding of CV1 to SARS-CoV-2 Spike RBD in the presence of human ACE2\u003cbr\u003e\nPlease note: This antibody was first isolated as an IgG1 lambda subclass. During the cloning process for the recombinant product, this was converted to an IgG1 kappa subclass, although the light chain variable region is still of the lambda subclass.\u003cbr\u003e\nab277514 is the carrier-free version of\u003cbr\u003e\nab277512\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe SARS-CoV-2 Spike Ectodomain often referred to simply as the spike protein is a critical component of the virus that enables entry into host cells. This glycoprotein is about 180 kDa and is prominently expressed on the viral surface. The spike protein is marked by significant regions including the S1 and S2 subunits which facilitate the binding and fusion processes necessary for viral entry into human cells. Variants like the D614G and notable mutations such as H69 deletion or D80A affect its structure. The spike protein is central to vaccine design due to its role in mediating infection.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe spike protein is critical for the interaction with the host angiotensin-converting enzyme 2 (ACE2) serving as an important component in the viral entry into human cells. It forms a trimeric complex important for mediating the fusion of the viral membrane with the host cell's membrane. Structural changes such as deletions in the HV69-70 region or mutations like D80A can impact the protein's functionality and viral virulence. Therefore understanding these mutations has implications for infection control efforts.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe spike protein plays a central role in the viral invasion pathway that starts with the recognition of the ACE2 receptor on the host cell. This pathway integrates the spike protein's functionality with other viral and host proteins leading to cell entry and subsequent viral replication. The renin-angiotensin system (RAS) is closely related given the ACE2 receptor's involvement in this physiological pathway highlighting the importance of these interactions in both viral pathology and host response.\u003cbr\u003e\nThe spike protein is instrumental in the pathogenesis of COVID-19 and related respiratory disorders. Its interaction with ACE2 is central in determining the infection's severity and transmission potential. Additionally the spike protein's structural changes such as HV69-70 deletions or D80A mutations can alter the disease manifestation by affecting how the immune system recognizes the virus. This structural variability potentially impacts vaccine efficacy and therapeutic antibody effectiveness complicating disease management and treatment strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854581420201,"sku":"ab277514","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab277514","provider":"Iright","version":"1.0","type":"link"}