{"product_id":"abcam-ab277859","title":"Abcam, ab277859, Human NF2 (Merlin) knockout Hep G2 cell line","description":"\u003cp\u003eSize: 2 x 1000000Cells \/ vial \/ 1000000Cells \/ vial\u003cbr\u003e\nNF2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR\/Cas9, functional Homozygous: 4 bp insertion and 1 bp insertion in exon 6. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:Hep G2,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Liver,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,\u003cbr\u003e\nMutation description:Knockout achieved by using CRISPR\/Cas9, functional Homozygous: 4 bp insertion and 1 bp insertion in exon 6,\u003cbr\u003e\nDisease:Hepatocellular Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-NF2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe NF2 gene produces a protein known as Neurofibromin 2 often called Merlin. The NF2 protein or Merlin protein plays a mechanical role in the cell as a tumor suppressor by stabilizing cellular structure and regulating cell growth. Its molar mass is approximately 70 kDa. NF2 protein is found mainly in the nervous system particularly in Schwann cells which are important for nerve function.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe NF2 protein functions to control cell proliferation differentiation and motility. It associates with the cytoskeleton acting as a bridge between cell surface proteins and the actin cytoskeleton. Merlin as part of the ERM (ezrin radixin moesin) family contributes to the assembly of protein complexes at the cell membrane. Through its interactions it plays a significant role in maintaining proper cell signaling and structural integrity.\u003cbr\u003e\nPathways\u003cbr\u003e\nNF2 protein integrates into the Hippo signaling pathway which regulates organ size by controlling cell growth and apoptosis. Within this pathway Merlin interacts with members such as YAP1 and LATS1\/2 moderating their activity to prevent over-proliferation of cells. NF2 also connects with the mTOR pathway which is critical for cell metabolism and growth further emphasizing its regulatory functions in cellular homeostasis.\u003cbr\u003e\nMutations or deletions in the NF2 gene are associated with neurofibromatosis type 2 a condition that causes benign tumors on nerve tissues known as schwannomas. NF2 protein is linked to other proteins such as angiogenesis factors contributing to the development of these tumors. Schwannomatosis another disorder related to NF2 involves nerve sheath tumor formation with Merlin mutation playing a role indicating its importance in nervous system health.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845124903081,"sku":"ab277859","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab277859","provider":"Iright","version":"1.0","type":"link"}