{"product_id":"abcam-ab279352","title":"Abcam, ab279352, Human C3a ELISA kit","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nHuman C3a ELISA kit is a sandwich ELISA designed to quantify Human C3a with a sensitivity of 63 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 62.5 - 4000 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Cell culture supernatant, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 63 pg\/mL,\u003cbr\u003e\nRange:62.5 - 4000 pg\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman C3a ELISA kit ab279352 is a sandwich ELISA to measure Human C3a in serum, plasma, cell culture supernatant with a sensitivity of 63 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThis assay employs an antibody specific for human C3a coated on a 96-well plate. Standards and samples are pipetted into the wells and C3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human C3a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of C3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman C3a ELISA kit ab279352 protocol summary\u003cbr\u003e\n1. Add standard or sample to each well used. Incubate at room temperature\u003cbr\u003e\n2. Wash and add prepared biotinylated antibody to each well. Incubate at room temperature.\u003cbr\u003e\n3. Wash and add prepared streptavidin solution. Incubate at room temperature.\u003cbr\u003e\n4. Add TMB Development Solution to each well. Incubate at room temperature.\u003cbr\u003e\n5. Add Stop Solution to each well. Read at 450nm immediately.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Complement Component 3 (C3) is a central protein in the complement system often referred to as C3 or C3a when discussing its active fragment. C3 weighs approximately 187 kDa and expresses widely in the liver then circulating in blood plasma. When activated C3 cleaves into C3a and C3b. C3a acts as an anaphylatoxin contributing to the immune response by promoting inflammation. C3 plays critical roles in immunity by enhancing phagocytosis and clearance of pathogens.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nC3 fragments particularly C3a influence various immune responses. As part of the complement system C3 integrates into a complex network of proteins. This activation cascade enhances the body's ability to combat pathogens. C3a interacts with receptors on immune cells mediating inflammatory response and acting as a chemoattractant. C3b another key fragment binds to pathogen surfaces aiding opsonization and immune clearance.\u003cbr\u003e\nPathways\u003cbr\u003e\nC3's function plays an important role in the alternative and classical complement pathways. In the alternative pathway C3b contributes directly by forming a C3 convertase complex. This interaction facilitates further cleavage of C3 amplifying immune response. C3b also associates with proteins like Factor B and Factor D to stabilize the convertase complex and enhance opsonization. In the classical pathway C3 interacts with components like C4b to optimize pathogen elimination.\u003cbr\u003e\nC3 and its fragments have significant connections to immune disorders and infections. Low levels or dysfunctional C3 can lead to susceptibility to bacterial infections. C3a has associations with asthma where it exacerbates the inflammatory response in lung tissues. In autoimmune diseases such as systemic lupus erythematosus (SLE) improper regulation of C3 activation leads to tissue damage. The interaction of C3 with proteins like C1q in SLE highlights the interplay between complement activation and autoimmune pathologies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843644641449,"sku":"ab279352","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab279352","provider":"Iright","version":"1.0","type":"link"}