{"product_id":"abcam-ab279781","title":"Abcam, ab279781, Phosphotyrosine Eph receptor A4\/SEK ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhosphotyrosine Eph receptor A4\/SEK ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine Eph receptor A4\/SEK in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospho-Eph receptor A4\/SEK ELISA Kit (ab279781) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Eph receptor A4\/SEK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human phospho-Eph receptor A4\/SEK. An anti-Eph receptor A4\/SEK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated Eph receptor A4\/SEK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-Eph receptor A4\/SEK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe Eph receptor A4 also known as SEK is a type of ephrin receptor that plays a significant role in cellular communication. It belongs to the Eph protein family and it is classified as a receptor tyrosine kinase. EphA4 has an approximate molecular mass of 110 kDa. It is mainly expressed in the nervous system although it can also be found in other tissues including the vascular and skeletal systems. This receptor has an important function in mediating signal transduction upon binding with their ephrin ligands.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nEphA4 plays a critical role in developmental processes particularly in the development of the nervous system and vascular patterning. The receptor engages in bidirectional signaling meaning that signals are transmitted not only into the receptor-expressing cells but also into the ephrin-expressing ones. EphA4 is part of complexes that can include multiple receptor and ephrin molecules which allows for the modulation of cell adhesion and migration. These processes are essential for forming tissue boundaries and axon guidance.\u003cbr\u003e\nPathways\u003cbr\u003e\nEphA4 signaling is involved in two significant pathways: the Eph\/ephrin signaling pathway and the cytoskeletal remodeling pathway. In the Eph\/ephrin signaling pathway EphA4 interacts with various ephrin ligands leading to changes in cellular architecture and behavior. The cytoskeletal remodeling pathway involves interactions with proteins such as Rho and Ras which coordinate cytoskeletal changes necessary for cell movement and positioning. These pathways are integral to processes like axon guidance and cell migration underpinning EphA4’s role in development.\u003cbr\u003e\nEphA4 has been implicated in conditions such as amyotrophic lateral sclerosis (ALS) and cancer metastasis. In the context of ALS EphA4's interaction with proteins like RhoA affects axonal repair and neuron survival contributing to the disease's progression. Regarding cancer EphA4 influences tumor growth and spread with its signaling pathways impacting cellular invasion and angiogenesis. Understanding these aspects of EphA4 activity assists in developing therapeutic strategies for these disorders.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855658143913,"sku":"ab279781","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab279781","provider":"Iright","version":"1.0","type":"link"}