{"product_id":"abcam-ab279786","title":"Abcam, ab279786, Phospho-Estrogen receptor alpha (Ser118) ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhospho-Estrogen receptor alpha (Ser118) ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-Estrogen receptor alpha (Ser118) in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospho-Estrogen receptor alpha (S118) ELISA Kit (ab279786) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Estrogen receptor alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human and phospho-Estrogen receptor alpha. An anti-pan Estrogen receptor alpha antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Estrogen receptor alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and rabbit anti-phospho-ER-alpha (S118) antibody is used to detect phosphorylated Estrogen receptor alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Estrogen receptor alpha (S118) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nEstrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.\u003cbr\u003e\nPathways\u003cbr\u003e\nERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.\u003cbr\u003e\nERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855660568745,"sku":"ab279786","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab279786","provider":"Iright","version":"1.0","type":"link"}