{"product_id":"abcam-ab279803","title":"Abcam, ab279803, Phosphotyrosine FRK ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhosphotyrosine FRK ELISA Kit is a Semi-quantitative ELISA for the measurement of Phosphotyrosine FRK in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhosphotyrosine FRK ELISA Kit (ab279803) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated FRK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human phospho-FRK. An anti-pan FRK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and FRK present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated FRK. After washing away unbound antibody, Streptavidin-Conjugated HRP is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FRK bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nFRK also known as FYN-related kinase or RAK is a non-receptor tyrosine kinase with a molecular mass of approximately 56 kDa. This protein is predominantly expressed in epithelial tissues including those of the breast kidney and liver. FRK displays substrate specificity and catalytic function by phosphorylating specific tyrosine residues on target proteins which influences cellular processes such as proliferation and differentiation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis kinase contributes to the regulation of cell cycle progression and apoptosis. FRK acts solo as well as part of larger signal transduction complexes. It plays a vital role in limiting uncontrolled cellular growth which is particularly significant in maintaining tissue homeostasis. FRK regulates various molecular interactions essential for cell adhesion and communication adding another layer of control over cell physiology.\u003cbr\u003e\nPathways\u003cbr\u003e\nFRK integrates into signaling pathways like the PI3K\/AKT and MAPK pathways. These pathways are critical for mediating responses to growth signals and stress conditions. FRK's activity modulates protein interactions with key molecules such as p53 and PTEN influencing downstream effects that control cell cycle checkpoints and apoptosis. By interacting with these pathways FRK helps to balance cell proliferation and survival in response to extracellular cues.\u003cbr\u003e\nFRK has implications in cancer development particularly in breast cancer. Altered FRK expression or activity has been associated with tumor progression and resistance to treatment. FRK interacts with proteins such as BRCA1 contributing to its role in DNA damage response and tumor suppression. Additionally FRK's aberrant activity links to certain forms of renal disorders where it impacts cellular responses in renal tissue often through modulation of E-cadherin and β-catenin in epithelial cells.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855647592617,"sku":"ab279803","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab279803","provider":"Iright","version":"1.0","type":"link"}