{"product_id":"abcam-ab279887","title":"Abcam, ab279887, Phospho-PDGFR beta (Y751) and Total PDGFR beta ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nPhospho-PDGFR beta (Y751) and Total PDGFR beta ELISA Kit is a Semi-quantitative ELISA for the measurement of Phospho-PDGFR beta (Y751) and Total PDGFR beta in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Semi-quantitative,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPhospho-PDGFR beta (Y751) and Total PDGFR beta ELISA Kit (ab279887) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated PDGFR beta protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.\u003cbr\u003e\nThis Sandwich ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the measurement of human phospho-PDGFR beta and total PDGFR beta. An anti-pan PDGFR beta antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and PDGFR beta present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho PDGFR beta (Y751) antibody is added to detect phosphorylated PDGFR beta. In the remaining wells, biotinylated anti-pan-PDGFR beta antibody is used to detect pan PDGFR beta. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGFR beta (Y751) or pan PDGFR beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nPDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.\u003cbr\u003e\nPDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855651655849,"sku":"ab279887","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab279887","provider":"Iright","version":"1.0","type":"link"}