{"product_id":"abcam-ab280099","title":"Abcam, ab280099, Human RAB29 knockout A549 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nRAB29 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-RAB29, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nRAB29 also known as Rab7L1 is a member of the RAB family of small GTPases. This protein is involved in vesicle trafficking processes within cells. RAB29 has an approximate mass of 23 kDa. It is expressed in various tissues but shows higher expression levels in brain and testis. In cellular functions it acts mainly within the endosomal-lysosomal system to regulate membrane trafficking events.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nRAB29 plays an important role in ensuring the proper transport and sorting of proteins within cells. It associates with certain effector proteins to form complexes which facilitate movement between endosomes and other intracellular compartments. It coordinates with other RAB proteins to maintain cellular homeostasis and ensure effective intracellular transport. This coordination is significant for neural cells suggesting a specific importance in neurological environments.\u003cbr\u003e\nPathways\u003cbr\u003e\nRAB29 engages in intracellular trafficking pathways that include the endocytic pathway and Golgi-lysosomal trafficking. In these pathways it cooperates closely with proteins like LRRK2 to regulate autophagy and the maintenance of the endosomal membrane system. Through these pathways it maintains cellular organelle distribution and affects lysosome functionality impacting overall cell health and metabolism.\u003cbr\u003e\nRAB29 exhibits connections to neurodegenerative disorders such as Parkinson’s disease. Mutations or dysfunctions in RAB29 along with its interaction partner LRRK2 have been linked to the pathogenesis of this disorder implicating it in the degeneration of dopaminergic neurons. Additionally aberrant RAB29 activity has been associated with complications in lysosomal storage diseases suggesting its broader implications in cellular waste handling and turnover.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845096853673,"sku":"ab280099","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab280099","provider":"Iright","version":"1.0","type":"link"}