{"product_id":"abcam-ab280114","title":"Abcam, ab280114, Mouse PRKAA1 knockout RAW 264.7 cell lysate","description":"\u003cp\u003eSize: 1Kit\u003cbr\u003e\nPRKAA1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:RAW 264.7,\u003cbr\u003e\nSpecies or organism:Mouse,\u003cbr\u003e\nTissue:Lymphatic,\u003cbr\u003e\nKnockout validation:Sanger Sequencing,Western blot,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nKnockout cell lysate achieved by CRISPR\/Cas9.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003cbr\u003e\nLysate preparation:\u003cbr\u003e\nOur lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).\u003cbr\u003e\nThis means that the protein of interest is denatured.\u003cbr\u003e\nIf you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.\u003cbr\u003e\nUser storage instructions:\u003cbr\u003e\nLyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-PRKAA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nPRKAA1 also known as the alpha 1 catalytic subunit of AMP-activated protein kinase (AMPK) plays an important role in cellular energy homeostasis. It has a molecular mass of approximately 63 kDa. The protein is widely expressed in various tissues including the liver skeletal muscle and brain. As a serine\/threonine kinase PRKAA1 reacts to changes in cellular energy status by activating pathways that restore energy balance.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nPRKAA1 acts as an energy sensor and regulator in cells. As part of the AMPK heterotrimeric complex it regulates energy metabolism by phosphorylating various substrates involved in lipid glucose and protein metabolism. PRKAA1 activation results in increased glucose uptake fatty acid oxidation and mitochondrial biogenesis while inhibiting the synthesis of fatty acids cholesterol and triglycerides. This protein plays a significant role in maintaining cellular energy homeostasis and adapting to metabolic stress.\u003cbr\u003e\nPathways\u003cbr\u003e\nPRKAA1 links to both the mTOR signaling pathway and the insulin signaling pathway. In the mTOR pathway it interacts with proteins such as TSC2 and Raptor leading to the inhibition of mTORC1 activity. In the insulin signaling pathway PRKAA1 affects the phosphorylation state of proteins involved in glucose homeostasis. PRKAA1’s regulation of these pathways highlights its importance in metabolic processes and energy balance.\u003cbr\u003e\nPRKAA1 shows association with metabolic disorders like type 2 diabetes and obesity. In type 2 diabetes dysregulation of PRKAA1-related pathways impacts insulin sensitivity and glucose uptake contributing to disease progression. Furthermore PRKAA1 relates to cardiac diseases often involving proteins such as PGC-1α which is important for mitochondrial biogenesis and energy metabolism in heart tissues. Understanding PRKAA1’s role offers insights into potential therapeutic targets for these metabolic diseases.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845073752233,"sku":"ab280114","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab280114","provider":"Iright","version":"1.0","type":"link"}