{"product_id":"abcam-ab281992","title":"Abcam, ab281992, Murine Interferon gamma ELISpot Pair","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nMurine Interferon gamma ELISpot Pair (ab281992) is part of the assay kits range. Abcam offers high-quality biological reagents and tools including antibodies, proteins, assays, cell lines and lysates.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Suspension cells,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:ELISpot,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMurine Interferon gamma ELISpot Antibody Pair (ab281992) is an antibody pair designed for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level by the ELISpot method.\u003cbr\u003e\nFor the quantitative determination of the frequency of Murine Interferon gamma-producing cells.\u003cbr\u003e\nFor research use only - not intended for diagnostic use.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nInterferon gamma (IFN-γ) also known as type II interferon is a cytokine that plays an important role in immune response. IFN-γ has a molecular weight of about 17 kDa and is produced by T cells and natural killer (NK) cells. IFN-γ binds to the interferon gamma receptor initiating a signaling cascade that activates various genes involved in immune functions. It is expressed mainly in activated immune cells within lymphoid tissues and inflamed sites during immune responses.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThis cytokine is significant in promoting macrophage activation enhancing the antigen presentation process and boosting the antimicrobial activity of phagocytes. IFN-γ is not part of a larger protein complex but works as a homodimer in signal transduction. Its production heightens the Th1 immune response by stimulating the differentiation of naïve T cells into Th1 cells which is essential for effective cellular immunity.\u003cbr\u003e\nPathways\u003cbr\u003e\nIFN-γ is integrally involved in the JAK-STAT signaling pathway alongside another critical cytokine Interleukin-12. This pathway further amplifies the immune response by regulating the expression of genes associated with cellular defense mechanisms. IFN-γ also interacts with the NF-kB pathway influencing inflammation and the activation of further immune responses. These interactions show a network of cooperativity with proteins like STAT1 and NF-kB essential for executing its biological roles.\u003cbr\u003e\nIFN-γ is linked to autoimmune diseases such as rheumatoid arthritis and multiple sclerosis where its elevated levels can exacerbate inflammatory processes. It connects to other proteins like TNF-alpha in promoting the inflammatory cascade. Moreover lower levels of IFN-γ are associated with a heightened risk of infections like tuberculosis demonstrating its vital role in pathogen defense. Therefore understanding IFN-γ and its interactions can be key in developing therapeutic approaches against these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843793670313,"sku":"ab281992","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab281992","provider":"Iright","version":"1.0","type":"link"}