{"product_id":"abcam-ab283434","title":"Abcam, ab283434, Recombinant Human Cathepsin B protein (Active)","description":"\u003cp\u003eSize: 10µg \/ 50µg \/ 100µg\u003cbr\u003e\nRecombinant Human Cathepsin B protein (Active) is a Human Full Length protein, in the 18 to 339 aa range with \u0026gt;=95% purity, \u0026lt;= 0.005 EU\/µg endotoxin level and suitable for Functional studies, SDS-PAGE, mass spectrometry and HPLC. The predicted molecular weight of ab155639 protein is 35.9 kDa. - Save time and ensure accurate results - use our Cathepsin B protein as a control - Optimal protein bioactivity, stability and reproducibility - Available in different sizes to fit your experimental needs\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt;0.005 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, Mass Spec, FuncS, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Fully biologically active determined by its ability to cleave the fluorogenic peptide substrate Z-Leu-Arg-AMC (Z-LR-AMC). The specific activity is comparable to the same protein (Human Cathepsin B\/CTSB) from other vendors.,\u003cbr\u003e\nAccession:P07858,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in PBS,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nEnsure the validity of your result using our recombinant human Cathepsin B protein ab283434 as a control.\u003cbr\u003e\nThe ab283434 Cathepsin B\/APPS protein is sourced from HEK293 cells and can be used as a positive control in SDS-PAGE, mass spectrometry and HPLC.\u003cbr\u003e\nCheck out our protein gel staining guide for SDS-PAGE\u003cbr\u003e\nPremium Bioactive Protein range\u003cbr\u003e\nThe ab283434 Cathepsin B\/APPS protein is part of the premium bioactive protein range which are ideal for preclinical cell culture and functional studies. These recombinant proteins are of the highest activity, purity, and consistency, meeting rigorous biophysical characterization.\u003cbr\u003e\nMore premium bioactive proteins can be found\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nCathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.\u003cbr\u003e\nCathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843811332265,"sku":"ab283434","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab283434","provider":"Iright","version":"1.0","type":"link"}