{"product_id":"abcam-ab284002","title":"Abcam, ab284002, Human GRX2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman GRX2 ELISA Kit is a Sandwich (qualitative) ELISA for the measurement of Human GRX2 in Human in Cell\/Tissue Extracts samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (qualitative),\u003cbr\u003e\nSensitivity:= 0.3 ng\/mL,\u003cbr\u003e\nRange:0.038 - 2.4 ng\/mL,\u003cbr\u003e\nAssay time:4h,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman GRX2 ELISA Kit (ab284002) is designed for detection of glutaredoxin-2 in human cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human glutaredoxin-2 in approximately 4 hours.\u003cbr\u003e\nA polyclonal antibody specific for human glutaredoxin-2 has been pre-coated onto a 96-well microplate with removable strips. Glutaredoxin-2 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human glutaredoxin-2, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe target GRX2 also commonly known as GLRX2 is a human protein encoded by the GLRX2 gene. GRX2 functions as a glutaredoxin a type of enzyme involved in the process of reducing disulfide bonds in proteins which is essential for cell redox regulation. The protein has a molecular mass of approximately 16 kDa and primarily resides in the mitochondria and cytoplasm of cells. Its expression occurs in various tissues with significant levels in the heart brain and skeletal muscle indicating its broad functional roles across different organ systems.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe GRX2 protein plays a critical role in maintaining cellular redox balance by facilitating the reversible oxidation of protein cysteines. This is important for protecting cells against oxidative stress. GRX2 does not function as an isolated entity but instead forms part of larger multi-protein complexes interacting with other enzymes to regulate cellular homeostasis. These interactions help to protect cells from oxidative damage promote protein folding and regenerate other antioxidants.\u003cbr\u003e\nPathways\u003cbr\u003e\nGRX2 holds a significant position in the redox regulation and antioxidant pathways. It partners with thioredoxin reductase and glutathione systems to mediate these pathways and manage cellular oxidative stress. The protein's activity is closely tied to glutathione with GRX2 often acting in synergy with glutathione to reduce complex oxidized protein thiols. This collaboration is vital in the oxidative stress response where the pathway elements must efficiently counterbalance oxidative challenges.\u003cbr\u003e\nGRX2's function links it to several oxidative stress-related conditions including neurodegenerative diseases and cardiovascular disorders. In neurodegenerative diseases like Parkinson's disease GRX2's ability to combat oxidative stress is compromised leading to neuronal damage. Additionally the protein's interaction with other redox regulatory proteins such as thioredoxin suggests interconnected roles in the progression of these disorders. Cardiovascular diseases also involve GRX2 due to its essential function in maintaining cardiovascular integrity under environmental and metabolic oxidative stress.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855675510953,"sku":"ab284002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab284002","provider":"Iright","version":"1.0","type":"link"}