{"product_id":"abcam-ab285291","title":"Abcam, ab285291, Mouse Estrogen ELISA Kit","description":"\u003cp\u003eSize: 96Test\u003cbr\u003e\nMouse Estrogen ELISA Kit is a Competitive ELISA used to quantify Mouse Estrogen with a sensitivity of 9.375 pg\/ml - Colorimetric ELISA kit - 450 nm readout. Works on any standard plate reader - Wide dynamic range – 15.625 - 1000 pg\/ml\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Homogenate, Cell culture supernatant, Serum, Other biological fluids,\u003cbr\u003e\nAssay type:Quantitative,\u003cbr\u003e\nSensitivity:\u0026lt; 9.375 pg\/mL,\u003cbr\u003e\nRange:15.625 - 1000 pg\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse Estrogen ELISA Kit is a competitive ELISA kit for the quantitative measurement of mouse estrogen with a sensitivity of 9.375 pg\/mL\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nMouse Estrogen ELISA kit an285291 is a competitive ELISA kit.\u003cbr\u003e\nThere are 2 types of competitive ELISAs:\u003cbr\u003e\n- \"antigen down\": The sample antigen competes with a reference antigen for binding to a specific amount of labeled antibody. The reference antigen is pre-coated on a multi-well plate. The sample is added to the plate wells with a labeled antibody. Depending on the amount of antigen in the sample, more or fewer free antibodies will be available to bind the reference antigen. This means that the more of the antigen there is in the sample, the less reference antigen will be detected and the weaker the signal will be.\u003cbr\u003e\n- \"antibody down\": the labeled antigen and the sample antigen (unlabeled) compete for binding to the primary antibody, which is immobilized on the plate. The lower the amount of antigen in the sample, the stronger the signal due to more labelled antigen in the well.\u003cbr\u003e\nAssay specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility.\u003cbr\u003e\nMouse Estrogen ELISA kit ab285291 protocol summary\u003cbr\u003e\n1. wash plate before adding standards, controls and samples\u003cbr\u003e\n2. Add standard, samples and controls into appropriate wells. Incubate 45min\u003cbr\u003e\n3. Wash\u003cbr\u003e\n4. Add SABC solution into each well. Incubate for 30 min\u003cbr\u003e\n5. Wash\u003cbr\u003e\n6. Add TMB substrate into each well. Incubate for 15-30 min\u003cbr\u003e\n7. Add stop solution. Read at 450 nm within 20 min.\u003cbr\u003e\nHow other researchers are using Mouse Estrogen ELISA kit ab285291\u003cbr\u003e\nMouse estrogen ELISA kit has been used to study the role of mitochondria dynamics in the female reproductive system in mice\u003cbr\u003e\nand to study the mechanism of the NKB-NK3R pathway in Polycystic ovary syndrome (PCOS).\u003cbr\u003e\nReferences\u003cbr\u003e\nYP Budi et al. PMID 36765080\u003cbr\u003e\nFei Guo et al. PMID 36453600\u003cbr\u003e\nRelated and Recommended products\u003cbr\u003e\nMouse estrogen ELISA kit ab285291 is commonly used to study the reproductive system in mice. Another kit to assess reproductive hormones in mice is\u003cbr\u003e\n- Mouse\/Rat testosterone ELISA kit\u003cbr\u003e\nab285350\u003cbr\u003e\nThis product is manufactured by BioVision, an Abcam company and was previously called K4265 Estrogen (Mouse) ELISA Kit. K4265-100 is the same size as the 96 test size of ab285291.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843636875433,"sku":"ab285291","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab285291","provider":"Iright","version":"1.0","type":"link"}