{"product_id":"abcam-ab288791","title":"Abcam, ab288791, Recombinant Mouse LIF protein (Active)","description":"\u003cp\u003eSize: 10µg \/ 50µg \/ 100µg\u003cbr\u003e\nRecombinant Mouse LIF protein (Active) is a Mouse Full Length protein, in the 25 to 203 aa range, expressed in HEK 293 cells, with \u0026gt;95%, \u0026lt;0.005 EU\/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nEndotoxin level:\u0026lt;0.005 EU\/µg,\u003cbr\u003e\nExpression system:HEK 293 cells,\u003cbr\u003e\nTags:Tag free,\u003cbr\u003e\nApplications:SDS-PAGE, Mass Spec, FuncSSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Fully biologically active determined by the dose dependent inhibition of M1 cells.ED50 is ≤ 0.22 ng\/ml, corresponding to a specific activity of 4.5 x 106 units\/mg.,\u003cbr\u003e\nAccession:P09056,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Mouse,\u003cbr\u003e\nReconstitution:Reconstitute in PBS,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLeukemia Inhibitory Factor (LIF) also known as LIF protein plays a critical mechanical role in cell communication and survival. It is a glycoprotein with a molecular weight (LIF MW) generally around 19 kilodaltons. In various tissues particularly in the mouse LIF expression occurs in the bone marrow muscle and notably the nervous system. Besides its presence in normal tissues it shows up in embryonic stem cells where it maintains their pluripotency in culture conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLIF influences cell differentiation growth and inflammatory response. As part of the interleukin-6 cytokine family LIF exerts its effects through a receptor complex involving LIFR (LIF receptor) and GP130. The LIF protein's signaling controls processes like neuronal cell differentiation and bone formation. It impacts hematopoietic cells and supports embryonic implantation and placental development by preparing the uterine environment.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe LIF protein is essential in the JAK\/STAT signaling pathway which regulates gene expression vital for survival and proliferation. It also interacts with the MAPK\/ERK pathway ensuring diverse cellular responses. These pathways link LIF to related proteins like STAT3 and ERK1\/2 which mediate further downstream effects. The interplay between these signaling cascades highlights LIF's role in managing cell survival and proliferation in various contexts.\u003cbr\u003e\nLIF contributes to physiological and pathological states. In cancer such as leukemia LIF influences tumor growth and cancer cell survival. The interaction with other cytokines like Interleukin-6 enhances its effect in oncogenic processes. In inflammatory diseases like rheumatoid arthritis LIF's role relates to inflammation and joint destruction. Understanding its interaction with inflammatory mediators can aid in targeted therapeutic strategies.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843854848169,"sku":"ab288791","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab288791","provider":"Iright","version":"1.0","type":"link"}