{"product_id":"abcam-ab288852","title":"Abcam, ab288852, Human JARID2 knockout A549 cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 2 x 1000000Cells \/ vial\u003cbr\u003e\nJARID2 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:A549,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Lung,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nKnockout validation:Next Generation Sequencing,\u003cbr\u003e\nDisease:Carcinoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlthough we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.\u003cbr\u003e\nWe will provide viable cells that proliferate on revival.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-JARID2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nJarid2 also known as Jumonji or Jumonji AT-rich interactive domain 2 serves important functions in chromatin dynamics. It is a protein with a mass of approximately 125 kDa and plays a role as a regulatory factor in gene expression. Jarid2 expresses across several tissues particularly in those undergoing developmental processes such as the heart and central nervous system. The protein helps to recruit other proteins to chromatin impacting the regulation of gene activity.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nJarid2 interacts with the polycomb repressive complex 2 (PRC2) which modulates chromatin structure to suppress gene transcription. This interaction is key for the protein's role in epigenetic regulation. As a component of PRC2 Jarid2 influences the methylation of histone H3 at lysine 27 (H3K27) a modification critical for controlling developmental genes' expression. Its function ensures proper regulation of gene programs that affect cell fate decisions during embryogenesis.\u003cbr\u003e\nPathways\u003cbr\u003e\nJarid2 integrates into the gene silencing pathways involving PRC2 influencing pathways like the Wnt\/β-catenin signaling pathway. This influence occurs by modulating target genes that PRC2 controls such as those involved in cellular differentiation. Additionally it relates to proteins such as Ezh2 another PRC2 member. Together these components maintain repression of genes impacting cell proliferation and differentiation.\u003cbr\u003e\nJarid2 proves significant in conditions like cardiac hypertrophy and certain cancers. Its deregulation may lead to altered expression of genes necessary for cardiac muscle cell size and function contributing to hypertrophy. In cancer changes in Jarid2 expression or function can disrupt normal growth control as seen in interactions with proteins like Suz12 another PRC2 associate. Understanding these connections helps to outline potential therapeutic targets for intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845164322985,"sku":"ab288852","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab288852","provider":"Iright","version":"1.0","type":"link"}