{"product_id":"abcam-ab289832","title":"Abcam, ab289832, Human Amyloid Beta 42 ELISA Kit","description":"\u003cp\u003eSize: 100Test\u003cbr\u003e\nHuman Amyloid Beta 42 ELISA Kit is a sandwich ELISA designed to quantify Human Amyloid Beta 42 with a sensitivity of 2.813 pg\/mL. - Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader - Wide dynamic range - quantifies 4.688 - 300 pg\/mL\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Plasma, Tissue Homogenate, Serum, Other biological fluids,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 2.813 pg\/mL,\u003cbr\u003e\nRange:4.688 - 300 pg\/mL,\u003cbr\u003e\nAssay Platform:Microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Amyloid Beta 42 ELISA Kit ab289832 is a sandwich ELISA to measure Human Amyloid Beta 42 in serum, plasma, tissue homogenate, other biological fluids with a sensitivity of 2.813 pg\/ml.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nThe density of color is proportional to the amount of human Amyloid Beta 42 captured from the samples.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details\u003cbr\u003e\nHuman Amyloid Beta 42 ELISA Kit ab289832 protocol summary\u003cbr\u003e\n1. Wash plate with wash solution\u003cbr\u003e\n2. Add standards and samples into appropriate wells. Incubate then discard\u003cbr\u003e\n3. Add Biotin detection antibody and incubate,\u003cbr\u003e\n4. Wash\u003cbr\u003e\nAdd SABC solution and incubate\u003cbr\u003e\n5. Wash\u003cbr\u003e\n6. Add TMB solution and incubate\u003cbr\u003e\n7. Add Stop solution and read at 450 nm\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nAmyloid beta 42 also known as Aβ42 is a peptide fragment generated through the proteolytic processing of the amyloid precursor protein (APP) by enzymes called beta-secretase and gamma-secretase. The mass of Aβ42 is approximately 4.5 kDa. It is primarily expressed in the central nervous system specifically in the brain where it plays a role in the normal synaptic function. Although in low concentrations Amyloid beta 42 is involved in a variety of neural activities.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nAmyloid beta 42 is an important component in the formation of amyloid plaques which are aggregates that occur extracellularly in the brain. These plaques are a major hallmark of Alzheimer's disease but generally Amyloid beta 42 can be found in both healthy and diseased brains. It is not part of a stable complex but it interacts with other types of amyloid beta peptides and possibly other molecular structures within the brain.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe production of Amyloid beta 42 is a central event in the amyloidogenic pathway which contributes to Alzheimer's disease pathogenesis. This pathway involves the sequential action of beta-secretase and gamma-secretase on APP. Aβ42 closely interacts with proteins like presenilin which are important components of the gamma-secretase complex influencing the proteolytic cleavage that determines the length of the amyloid beta peptides produced.\u003cbr\u003e\nAmyloid beta 42 is most notably linked to Alzheimer's disease where it contributes to neuronal death and cognitive decline along with its strong association with amyloid plaques. Some evidence also indicates its involvement in other neurodegenerative diseases like cerebral amyloid angiopathy. In Alzheimer's disease high levels of Amyloid beta 42 correlate with hyperphosphorylation of tau proteins another neuropathological feature which further drives the neurodegenerative process.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46855626129577,"sku":"ab289832","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab289832","provider":"Iright","version":"1.0","type":"link"}