{"product_id":"abcam-ab290979","title":"Abcam, ab290979, Mouse WISP1 ELISA Kit - Intracellular","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMouse WISP1 ELISA Kit - Intracellular is a single-wash 90-min Simplestep used to quantify Mouse WISP1 with a sensitivity of 10.517 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 10.517 pg\/mL,\u003cbr\u003e\nRange:31.25 - 2000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMouse WISP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of WISP1 protein in mouse cell extract. Quantitate Mouse WISP1 with 10.517 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nWISP1 also known as Wnt1 Inducible Signaling Pathway Protein 1 or CCN4 is a protein with significant roles in cellular processes. This protein has an approximate mass of 41 kDa. You can find its expression in various tissues including bone cartilage and vascular tissues. WISP1 belongs to the CCN family of proteins which are matricellular proteins that interact with different cell surface molecules and receptor systems to modulate cellular functions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nWISP1 plays an important role in cellular proliferation differentiation and survival. It functions as a matricellular protein that can form complexes with integrins and other cell surface receptors. This interaction influences cellular responses to environmental signals. WISP1 contributes to extracellular matrix interaction and cellular adhesion. By regulating these processes WISP1 supports tissue development repair and regeneration.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe network of interactions influenced by WISP1 impacts several key signaling pathways including the Wnt signaling pathway and the TGF-beta pathway. These pathways are important for regulating cell growth and differentiation. In connection with the Wnt pathway WISP1 interacts with proteins like beta-catenin which plays an important role in signal transduction and gene expression. These interactions help maintain proper cellular function and structural integrity.\u003cbr\u003e\nWISP1 shows links to cancer and fibrosis. Dysregulation of WISP1 expression and its related pathways can contribute to the development and progression of these conditions. In cancers WISP1 interacts with proteins like beta-catenin and contributes to the regulation of oncogenic processes such as tumor growth and metastasis. In fibrosis WISP1 influences the deposition of extracellular matrix proteins affecting tissue scarring and organ function.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843578122409,"sku":"ab290979","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab290979","provider":"Iright","version":"1.0","type":"link"}