{"product_id":"abcam-ab291253","title":"Abcam, ab291253, Recombinant human GAPDH protein (Active)","description":"\u003cp\u003eSize: 10µg \/ 50µg\u003cbr\u003e\nRecombinant human GAPDH protein (Active) is a Human Full Length protein, in the 1 to 335 aa range, expressed in Escherichia coli, with \u0026gt;95%, suitable for SDS-PAGE, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nPurity:\u0026gt;95% SDS-PAGE,\u003cbr\u003e\nExpression system:Escherichia coli,\u003cbr\u003e\nTags:His tag N-Terminus,\u003cbr\u003e\nApplications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Specific activity is ≥ 100 U\/mg when assayed in 100 mM Hepes pH 8.0, 10 mM NaH2PO4, 0.1% NP-40 at 37°C for 30 min.1 unit of GAPDH is the amount of enzyme that will catalyze 1 μmol of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate at pH 8.0 and 37°C.,\u003cbr\u003e\nAccession:P04406,\u003cbr\u003e\nAnimal free:No,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Human,\u003cbr\u003e\nReconstitution:Reconstitute in 50 mM Hepes and 10% glycerol to a final concentration of 1 mg\/ml. Store at -20, or aliquoted and stored at -70 for up to 2 years. For 1-2 weeks it is stable at 4C.,\u003cbr\u003e\nStorage buffer:pH: 7.4Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlyceraldehyde-3-phosphate dehydrogenase commonly known as GAPDH is an enzyme involved in glycolysis. Its molecular weight (MW) is approximately 36 kDa. The protein is expressed ubiquitously in almost all tissues reflecting its essential role in energy production. GAPDH catalyzes the sixth step of glycolysis converting glyceraldehyde-3-phosphate into 13-bisphosphoglycerate. Due to its stable expression researchers often use GAPDH as a loading control in western blot experiments.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGAPDH serves important metabolic functions beyond its enzymatic role in glycolysis. It functions as part of a multi-enzyme complex within the cytoplasm which facilitates efficient substrate channeling during glycolysis. Additionally GAPDH has non-glycolytic roles including involvement in nuclear processes like RNA export and DNA repair. Its ubiquitous presence across different cellular compartments indicates its multiple functions beyond metabolic pathways.\u003cbr\u003e\nPathways\u003cbr\u003e\nGAPDH integrates into significant cellular functions like the glycolytic pathway and apoptotic pathways. In glycolysis GAPDH collaborates with enzymes like phosphoglycerate kinase forming a cohesive link in the energy conversion chain. Its participation in apoptotic pathways highlights GAPDH's involvement in cellular death processes interacting with proteins like Bcl-2 to influence apoptosis progression. These roles reinforce its presence in central metabolic and regulatory pathways.\u003cbr\u003e\nGAPDH has associations with neurodegenerative diseases and cancer. In neurodegenerative disorders such as Alzheimer's disease GAPDH’s altered enzymatic activity is frequently observed influencing cellular energy homeostasis. Moreover overexpression or aberrant regulation of GAPDH relates to cancer cell proliferation and metastasis implicating proteins like p53 in these pathways. The diverse functions and interactions of GAPDH emphasize its importance in both normal cellular function and disease states.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843958689961,"sku":"ab291253","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab291253","provider":"Iright","version":"1.0","type":"link"}