{"product_id":"abcam-ab300512","title":"Abcam, ab300512, Anti-Glycosylated Robo4 antibody [EPR26283-158] (BSA and Azide free)","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal ROBO4 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR26283-158,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.,\u003cbr\u003e\nSpecificity:Robo4 is a glycoprotein and can be deglycosylated by Protein Deglycosylation MIX II (PMID: 19139490). This antibody does not recognize the deglycosylated form of Robo4. See WB image for details.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nGlycosylated Robo4 also known as Roundabout homolog 4 or Magic Roundabout is a transmembrane receptor with a molecular mass of approximately 100 kDa. This protein is predominantly expressed in the endothelial cells of blood vessels. Glycosylation plays a role in its function and stability. Robo4's principal function is to mediate cellular communication and contribute to the regulation of angiogenesis by interacting with other proteins and extracellular matrices.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nGlycosylated Robo4 regulates endothelial cell migration and vessel stability. It is not part of a large complex but collaborates with other proteins to maintain balanced blood vessel growth and repair. This activity provides a means for the vascular system to respond appropriately to cues for new vasculature or stabilize existing vessels in reaction to physiological demands.\u003cbr\u003e\nPathways\u003cbr\u003e\nGlycosylated Robo4 interacts within the angiogenesis signaling pathway and the Slit-Robo signaling pathway. Within these pathways Robo4 associates with proteins like Slit2 and recruits downstream signaling partners to mediate endothelial integrity and angiogenic responses. Its role ensures that both new vessel formation and remodeling of existing vessels are tightly regulated preventing abnormal sprouting or regression.\u003cbr\u003e\nGlycosylated Robo4 shows connections to pathologies such as cancer and diabetic retinopathy. In the context of cancer Robo4's interaction with Slit2 and other signaling proteins affects tumor angiogenesis by preventing excessive vessel growth that tumors could exploit for nutrition and spread. Similarly in diabetic retinopathy dysregulation of Robo4 activity can result in aberrant vessel formation in the retina leading to vision complications.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851779526825,"sku":"ab300512","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab300512","provider":"Iright","version":"1.0","type":"link"}