{"product_id":"abcam-ab302757","title":"Abcam, ab302757, Human LAMP2 ELISA Kit","description":"\u003cp\u003eSize: 96Tests\u003cbr\u003e\nHuman LAMP2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LAMP2 with a sensitivity of 18.248 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Validated on a number of sample types including cerebrospinal fluid (CSF) - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture media,\u003cbr\u003e\nReacts with:Human, Monkey,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 18.248 pg\/mL,\u003cbr\u003e\nRange:46.875 - 3000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman LAMP2 ELISA Kit ab302757 is a rapid single-wash 90-min Sandwich ELISA to measure Human LAMP2 in cell culture media, CSF, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 18.248 pg\/mL.\u003cbr\u003e\nHow the assay works\u003cbr\u003e\nHuman LAMP2 SimpleStep ELISA\u003cbr\u003e\nemploys capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details.\u003cbr\u003e\nAssay Specificity\u003cbr\u003e\nOur SimpleStep ELISA\u003cbr\u003e\nkits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.\u003cbr\u003e\nPlease refer to our protocol booklet for more details.\u003cbr\u003e\nHuman LAMP2 ELISA Kit ab302757 protocol summary\u003cbr\u003e\n1. Mix: add samples\/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature\u003cbr\u003e\n2. Wash\u003cbr\u003e\n3. Add TMB development solution - incubate for 10 min\u003cbr\u003e\n4. Add Stop solution\u003cbr\u003e\n5. Read the results on a plate reader at 450 nm\u003cbr\u003e\nDesign your own immunoassay\u003cbr\u003e\nWe offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:\u003cbr\u003e\n- Anti-LAMP2 antibody [YCA-R24444-31 H2L1] - BSA and Azide free (Capture)\u003cbr\u003e\nab288624\u003cbr\u003e\n- Anti-LAMP2 antibody [YCA-R24444-155 H2L1] - BSA and Azide free (Detector)\u003cbr\u003e\nab288624\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nLAMP2 also known as CD107b is an important protein found in the lysosomal membrane. It plays a significant role in lysosome function facilitating autophagy and the degradation of cellular debris. LAMP2 proteins are expressed in most tissues especially in tissues with high lysosomal activity like the liver kidney and heart. The LAMP2 molecule has a molecular weight of approximately 45 kDa. Researchers frequently use LAMP2 staining and LAMP2 marker to study its cellular localization and expression levels.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nLAMP2 is essential for the normal fusion of lysosomes with autophagosomes. This process ensures the recycling of cellular components and maintenance of cellular homeostasis. LAMP2 acts in conjunction with other members of the lysosome-associated membrane protein family LAMP1 and LAMP3 forming part of a larger complex. This interaction is fundamental in managing the degradation pathways within the cell supporting processes such as lipid metabolism and protein turnover.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe activity of LAMP2 is critical in the autophagic and degradation pathways within the cell. It particularly interacts in the autophagy-lysosome pathway where it aids the clearance of damaged organelles and protein aggregates. LAMP2 interaction with proteins such as the autophagy-related protein LC3 helps this degradation process. Additionally LAMP2 plays a role in endocytic pathways linking it to proteins involved in vesicle trafficking and membrane fusion.\u003cbr\u003e\nMutations or deficiencies in LAMP2 are associated with Danon disease a type of lysosomal storage disorder. Patients with Danon disease exhibit symptoms such as cardiomyopathy skeletal myopathy and intellectual disabilities. LAMP2 is also implicated in other lysosomal disorders with a connection to the malfunction of autophagy processes. Its dysfunction is often linked with proteins responsible for cellular degradation affecting the normal homeostatic balance and leading to the accumulation of autophagic substrates.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843566358697,"sku":"ab302757","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab302757","provider":"Iright","version":"1.0","type":"link"}