{"product_id":"abcam-ab302759","title":"Abcam, ab302759, Human MEF2C ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman MEF2C ELISA Kit is a single-wash 90-min Simplestep used to quantify Human MEF2C with a sensitivity of 65.614 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell Lysate,\u003cbr\u003e\nReacts with:Human, Rat,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 65.614 pg\/mL,\u003cbr\u003e\nRange:234.375 - 15000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman MEF2C SimpleStep ELISA\u003cbr\u003e\nkit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MEF2C protein in human cell extract. Quantitate Human MEF2C with 65.614 pg\/ml sensitivity.\u003cbr\u003e\nSimpleStep ELISA\u003cbr\u003e\ntechnology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA\u003cbr\u003e\nplates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA\u003cbr\u003e\nprotocol summary in the image section for further details. Our SimpleStep ELISA\u003cbr\u003e\ntechnology provides several benefits:\u003cbr\u003e\n-Single-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA\u003cbr\u003e\nmicroplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA\u003cbr\u003e\nREACH authorisation\u003cbr\u003e\nAbcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.\u003cbr\u003e\nIt is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThe MEF2C protein also known as MADS box transcription enhancer factor 2 polypeptide C plays an important role in gene expression regulation. This protein has a mass of approximately 52 kDa and is mainly found in the heart skeletal muscle brain and endothelial cells. It is part of the MEF2 family which includes other members like MEF2A MEF2B and MEF2D. MEF2C acts as a transcription factor binding to specific DNA sequences to activate the transcription of target genes involved in various biological processes.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe MEF2C protein is essential in cellular differentiation and development. MEF2C is not part of a complex but interacts with other cofactors and regulatory proteins to fulfill its roles. It is important in the development of cardiac and skeletal muscle cells and plays a significant role in neurogenesis and neuronal connectivity in the central nervous system. This protein's precise regulation ensures proper development and function of these tissues and organs.\u003cbr\u003e\nPathways\u003cbr\u003e\nMEF2C influences cellular signaling through the MAPK\/ERK and calcium\/calmodulin-dependent protein kinase pathways. In the MAPK\/ERK pathway MEF2C modulates gene expression in response to external stimuli interacting with proteins like ERK1\/2. In the calcium\/calmodulin-dependent kinase pathway MEF2C plays a role in calcium signaling networks impacting neuronal plasticity and survival. These pathways highlight MEF2C’s involvement in muscle and brain function.\u003cbr\u003e\nMEF2C mutations or dysregulation link to conditions like dilated cardiomyopathy and neurodevelopmental disorders such as MEF2C haploinsufficiency syndrome. In cardiac disease abnormal MEF2C activity can affect heart muscle cell function often involving proteins like actin and myosin. In neurodevelopmental disorders MEF2C's interaction with various transcriptional regulators impacts neuronal networks contributing to symptoms like impaired cognitive development and autism spectrum-like behaviors.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843574419625,"sku":"ab302759","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab302759","provider":"Iright","version":"1.0","type":"link"}