{"product_id":"abcam-ab303448","title":"Abcam, ab303448, ioGlutamatergic Neurons HTT 50CAG\/WT - Human iPSC derived cells","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 5000000Cells \/ vial\u003cbr\u003e\nioGlutamatergic Neurons HTT 50CAG\/WT - Human iPSC derived cells available to order. Wild type ioGlutamatergic Neurons (ab303447) form the genetically matched control for the ioGlutamatergic Neurons HTT50CAG\/WT disease model.\u003cbr\u003e\nKey facts\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nForm:LiquidSee storage information\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWild type ioGlutamatergic Neurons (\u003cbr\u003e\nab303447\u003cbr\u003e\n) form the genetically matched control for the ioGlutamatergic Neurons HTT\u003cbr\u003e\n50CAG\/WT\u003cbr\u003e\ndisease model. This physiologically-relevant isogenic pairing offers a powerful next generation model to study Huntington's disease in research and drug discovery.\u003cbr\u003e\nioGlutamatergic Neurons HTT\u003cbr\u003e\n50CAG\/WT\u003cbr\u003e\nare ioGlutamatergic Neurons carrying the disease-relevant 50 CAG trinucleotide repeat expansion, associated with Huntington's disease. ioGlutamatergic Neuron HTT\u003cbr\u003e\n50CAG\/WT\u003cbr\u003e\nhave been reprogrammed from human iPSCs using opti-ox technology, a precise reprogramming technology. Using CRISPR\/Cas9 genome editing an abnormal expansion of 50 CAG repeats has been introduced in the first exon of the Huntingtin gene. Human stem cells, within days, convert consistently into mature, functional glutamatergic neurons providing a high quality human model for the study of Huntington's disease.\u003cbr\u003e\nioGlutamatergic Neurons HTT\u003cbr\u003e\n50CAG\/WT\u003cbr\u003e\nexpress pan-neuronal and glutamatergic markers TUBB3, MAP2 and VGLUT2 by day 11, as well as the disease-relevant Huntingtin protein.\u003cbr\u003e\nThis disease model offers a fast and easy-to-use system for investigations into the impact of gene function on disease progression against an isogenic control.\u003cbr\u003e\nIn partnership with bit.bio\u003cbr\u003e\nKaryotype:\u003cbr\u003e\nNormal\u003cbr\u003e\nSeeding Density:\u003cbr\u003e\n30,000 cells\/cm\u003cbr\u003e\nSeeding compatibility:\u003cbr\u003e\n6-, 12-, 24-, 96- and 384-well compatible\u003cbr\u003e\nQuality control:\u003cbr\u003e\nICC and gene expression analysis\u003cbr\u003e\nResearch applications:\u003cbr\u003e\nAcademic research, Drug development, Neurotoxicology, Genetic screening (e.g. CRISPR screening).\u003cbr\u003e\nThis product is subject to limited use licenses from iPS Academia Japan Inc, TET Systems GmbH, ERS Genomics Limited and Sigma-Aldrich Co. LLC and is developed with Bit Bio patented technology. For full details of the licenses and patents please refer to our\u003cbr\u003e\nlimited use license and patent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46845055402153,"sku":"ab303448","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab303448","provider":"Iright","version":"1.0","type":"link"}