{"product_id":"abcam-ab306775","title":"Abcam, ab306775, Human SFRP1 knockout U-87 MG cell line","description":"\u003cp\u003eSize: 1000000Cells \/ vial \/ 2 x 1000000Cells \/ vial\u003cbr\u003e\nSFRP1 KO cell line available to order. KO validated by. Free of charge wild type control available. To order both knockout and wild-type control cells: select 2 x 1000000Cells\/vial. To order only knockout cells: select 1000000Cells\/vial.\u003cbr\u003e\nKey facts\u003cbr\u003e\nCell type:U-87 MG,\u003cbr\u003e\nSpecies or organism:Human,\u003cbr\u003e\nTissue:Brain,\u003cbr\u003e\nForm:LiquidSee storage information,\u003cbr\u003e\nDisease:Glioblastoma\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nAlthough we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.\u003cbr\u003e\nThis product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our\u003cbr\u003e\nlimited use license\u003cbr\u003e\npatent pages\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nGene name-SFRP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nSFRP1 also known as Secreted Frizzled-Related Protein 1 is a protein that functions mechanically by modulating Wnt signaling pathways. This protein weighs approximately 35 kiloDaltons. SFRP1 acts as a modulator by binding to Wnt ligands preventing them from interacting with their cell surface receptors. It is expressed in various tissues including the kidney retina lung and heart.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe secreted glycoprotein SFRP1 plays a role in cellular processes such as cell growth differentiation and apoptosis. As a member of the soluble frizzled-related proteins it acts to inhibit Wnt signaling pathways which are critical in maintaining cellular homeostasis. SFRP1 commonly interacts in the extracellular environment where it forms part of a regulatory complex that includes other extracellular matrix components to achieve its function.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe role SFRP1 plays is important in the Wnt signaling pathway and the regulation of β-catenin activity. These pathways are involved in embryonic development and tissue regeneration. SFRP1 directly interacts with other proteins like frizzled receptors and Wnt ligands to maintain balance in signaling. This modulation further influences gene expression patterns and cellular behavior.\u003cbr\u003e\nAn imbalance or alteration in SFRP1 activity is associated with cancer and osteoporosis. Its underexpression or lack of function can lead to abnormal cellular proliferation which is linked to tumorogenesis. In cancer SFRP1's interaction with the Wnt signaling pathway and its related proteins such as β-catenin highlights its role in maintaining normal cell function. Abnormal regulation also impacts bone density where SFRP1's regulatory function on Wnt S signaling may affect bone formation and lead to osteoporosis.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46847391203497,"sku":"ab306775","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab306775","provider":"Iright","version":"1.0","type":"link"}