{"product_id":"abcam-ab307103","title":"Abcam, ab307103, Human CD5 Antibody Pair - BSA and Azide free","description":"\u003cp\u003eSize: 10 x 96Tests\u003cbr\u003e\nHuman CD5 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CD5.\u003cbr\u003e\nKey facts\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:ELISA set,\u003cbr\u003e\nRange:15.6 - 1000 pg\/mL,\u003cbr\u003e\nAssay Platform:Reagents\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman CD5 Antibody Pair - BSA and Azide free (ab307103) is a matched pair of unconjugated\u003cbr\u003e\nrecombinant rabbit monoclonal\u003cbr\u003e\ncapture and detection antibodies used to (quantify human CD5) in sandwich ELISAs and many other pair-based applications.\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nUse our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with \u0026lt;1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nThe pair can be used in variety of assays and platforms including but not limited to:\u003cbr\u003e\nSandwich ELISA\u003cbr\u003e\nFRET\/TR-FRET\/HTRF\u003cbr\u003e\nMeso Scale Discovery\u003cbr\u003e\nBead-based assays\u003cbr\u003e\nAlphaLISA\u003cbr\u003e\n\/AlphaScreen\u003cbr\u003e\nDELFIA\u003cbr\u003e\nimmunoassays\u003cbr\u003e\nand Single Molecule Counting (SMC™) immunoassays\u003cbr\u003e\nMultiplex\u003cbr\u003e\nOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:\u003cbr\u003e\nBuffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.\u003cbr\u003e\nConcentration of ~1 mg\/ml as measured by the protein A280 method.\u003cbr\u003e\nWe can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.\u003cbr\u003e\nPairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.\u003cbr\u003e\nPlease note:\u003cbr\u003e\nThe recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.\u003cbr\u003e\nThe range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.\u003cbr\u003e\nWe guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.\u003cbr\u003e\nAntibody properties:\u003cbr\u003e\nCapture antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) – 100 µg\u003cbr\u003e\nDetector antibody:\u003cbr\u003e\nrecombinant rabbit monoclonal (unconjugated) - 100 µg\u003cbr\u003e\nConcentration\u003cbr\u003e\n: ~1 mg\/ml\u003cbr\u003e\nStorage buffer:\u003cbr\u003e\n100% PBS\u003cbr\u003e\nLiquid\u003cbr\u003e\nIsotype:\u003cbr\u003e\nRecombinant monoclonal antibodies offer several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free production\u003cbr\u003e\nMeso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.\u003cbr\u003e\nAlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.\u003cbr\u003e\nSimoa is a registered trademark of Quanterix, Inc.\u003cbr\u003e\nSMC is a registered trademark of Merck KGaA, Darmstadt, Germany.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD5 also known as T1 or Leu-1 is a glycoprotein with a molecular mass of approximately 67 kDa. It is expressed mainly on the surface of T cells and a subset of B cells. CD5 plays a major role in the regulation of immune responses. It functions by modulating T-cell receptor (TCR) signaling often acting as a negative regulator to prevent excessive immune activation. Researchers often utilize CD5 antibodies such as those conjugated with PerCP for immunohistochemistry (IHC) to study this protein's expression and distribution.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD5 is essential in the immune system's ability to maintain tolerance to self-antigens thereby preventing autoimmune responses. CD5 forms part of a receptor complex on the cell surface that interacts with ligands and other receptors to fine-tune immune cell signaling. Its localization and function in T cells relate closely to its ability to modulate signaling pathways essential for cell survival and proliferation. This modulation is important for ensuring that immune responses are appropriate to the stimuli encountered.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD5 is involved in signal transduction processes important for immune tolerance and modulation. It intersects with pathways like the T cell receptor (TCR) signaling pathway and the NF-kB signaling pathway. These pathways involve interaction with proteins like Zap70 a tyrosine kinase related to TCR signaling and the downstream activation of transcription factors that regulate immune responses. The protein CRIS1 also appears in some pathway interactions highlighting the interconnected nature of immune regulatory proteins.\u003cbr\u003e\nCD5 has significant associations with autoimmune diseases such as systemic lupus erythematosus (SLE) where its modulatory role can affect autoantibody production. Additionally CD5 in conjunction with proteins like OX19 correlates with certain lymphoproliferative disorders including chronic lymphocytic leukemia (CLL). In these disorders the expression and function of CD5 may impact disease progression and patient response to therapy making it a potential target for intervention in immune-related conditions.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843603026089,"sku":"ab307103","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab307103","provider":"Iright","version":"1.0","type":"link"}