{"product_id":"abcam-ab307566","title":"Abcam, ab307566, Anti-5-methyl cytosine adenosine di-nucleoside (5-mCpA) antibody [2C8H8A6] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nMouse Recombinant Monoclonal 5-mCpA antibody. Carrier free. Suitable for Dot and reacts with Modified Nucleic Acid samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Mouse,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:2C8H8A6,\u003cbr\u003e\nIsotype:IgG1,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nApplications:DotSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003cbr\u003e\nWant a custom formulation?\u003cbr\u003e\nThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\n5-Methyl cytosine adenosine dinucleoside (5-mCpA) functions mechanically as an epigenetic modification. It constitutes a specific sequence where the cytosine base undergoes methylation forming a methyl group at the 5th carbon position. This modification takes place largely in genomic DNA where cytosine nucleosides occur beside adenosine nucleosides. 5-mCpA commonly appears in various cell types with higher expression regions observed specifically in gene regulatory domains in eukaryotic cells. The mass of 5-mCpA can differ depending on the arranged nucleotide framework containing the methyl group.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThe mechanism involving this dinucleoside affects gene expression regulation. 5-mCpA functions as part of a complex involved in chromatin remodeling. Additionally this complex interacts with other modified nucleotides such as 5-methyl cytosine. Its role is to influence the transcriptional activation or repression of associated genes by altering structural proteins that bind to the chromatin. The presence of these methylated nucleosides including methyl azide-modified versions indicates an important control mechanism in developmental processes and differentiation within organisms.\u003cbr\u003e\nPathways\u003cbr\u003e\n5-mCpA plays an essential part in epigenetic regulation and the DNA methylation pathway. The DNA methylation pathway runs in parallel with histone modification to alter chromatin structure and gene accessibility. Proteins such as DNA methyltransferases are closely related to the formation and maintenance of 5-mCpA within these pathways. The interaction between methylation paths and transcription factors contributes significantly to controlling cell cycle progression and other cellular processes.\u003cbr\u003e\nAlterations in 5-mCpA levels directly associate with cancer and neurological disorders. Aberrant DNA methylation patterns involving 5-mCpA can contribute to tumorigenesis as seen in certain cancer types where loss of methylation is observed. Similarly neurological conditions like Rett syndrome may relate to disruptions in this methylation process. Proteins like MECP2 which bind specifically to methylated DNA including 5-mCpA are implicated in these disorder pathways indicating a connection between altered epigenetic marks and disease manifestation.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46854772031657,"sku":"ab307566","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab307566","provider":"Iright","version":"1.0","type":"link"}