{"product_id":"abcam-ab309482","title":"Abcam, ab309482, Anti-TET2 antibody [EPR26694-93] - BSA and Azide free","description":"\u003cp\u003eSize: 100µg \/ 1mg\u003cbr\u003e\nRabbit Recombinant Monoclonal TET2 antibody. Carrier free. Suitable for WB, ICC\/IF, Flow Cyt (Intra), IP and reacts with Mouse samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nHost species:Rabbit,\u003cbr\u003e\nClonality:Monoclonal,\u003cbr\u003e\nClone number:EPR26694-93,\u003cbr\u003e\nIsotype:IgG,\u003cbr\u003e\nCarrier free:Yes,\u003cbr\u003e\nReacts with:Mouse,\u003cbr\u003e\nApplications:IP, Flow Cyt (Intra), ICC\/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nImmunogen:The exact immunogen used to generate this antibody is proprietary information.\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nPatented technology\u003cbr\u003e\nOur RabMAb\u003cbr\u003e\ntechnology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to\u003cbr\u003e\nRabMAb® patents\u003cbr\u003e\nWhat are the advantages of a recombinant monoclonal antibody?\u003cbr\u003e\nThis product is a recombinant monoclonal antibody, which offers several advantages including:\u003cbr\u003e\n- High batch-to-batch consistency and reproducibility\u003cbr\u003e\n- Improved sensitivity and specificity\u003cbr\u003e\n- Long-term security of supply\u003cbr\u003e\n- Animal-free batch production\u003cbr\u003e\nFor more information, read more on\u003cbr\u003e\nrecombinant antibodies\u003cbr\u003e\nConjugation ready\u003cbr\u003e\nOur carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.\u003cbr\u003e\nUse our\u003cbr\u003e\nconjugation kits\u003cbr\u003e\nfor antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.\u003cbr\u003e\nCompatibility\u003cbr\u003e\nThis product is compatible with the Maxpar\u003cbr\u003e\nAntibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar\u003cbr\u003e\nis a trademark of Fluidigm Canada Inc.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nForm-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nTet2 also known as ten-eleven translocation methylcytosine dioxygenase 2 is an enzyme involved in DNA demethylation. It has a molecular weight of approximately 224 kDa. This enzyme catalyzes the conversion of 5-methylcytosine to 5-hydroxymethylcytosine an important step in DNA demethylation processes. Tet2 expression occurs in various tissues with higher levels in hematopoietic cells. Its role in modification of DNA has made it a significant subject of study in epigenetic regulation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nTet2 participates in the regulation of gene expression through its impact on DNA methylation status. By promoting DNA demethylation Tet2 influences transcriptional networks that are vital for cell differentiation and development. The enzyme functions as part of a larger complex with cofactors including ascorbic acid which it requires for full enzymatic activity. Its actions affect the regulation of important processes such as self-renewal and differentiation in hematopoietic stem cells.\u003cbr\u003e\nPathways\u003cbr\u003e\nThe enzymatic activity of Tet2 plays a role in epigenetic pathways involved in hematopoiesis and immune function. In particular Tet2 interacts with pathways regulating chromatin structure and transcriptional control. It associates with proteins like TET1 and TET3 as they share roles in the regulation of 5-hydroxymethylcytosine levels. Through these mechanisms Tet2 contributes significantly to the maintenance of normal hematopoietic and immune cell function.\u003cbr\u003e\nAlterations in Tet2 activity have been linked to hematological malignancies such as myelodysplastic syndromes and acute myeloid leukemia. Loss of function mutations in Tet2 lead to disruptions in hematopoietic differentiation contributing to disease pathology. Tet2 mutations can affect interactions with proteins such as DNMT3A another promoter of DNA methylation which further influence the disease course. Understanding Tet2's role in these disorders may offer potential therapeutic insights.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46851747578025,"sku":"ab309482","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab309482","provider":"Iright","version":"1.0","type":"link"}