{"product_id":"abcam-ab313510","title":"Abcam, ab313510, Human ASAH2 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman ASAH2 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human ASAH2 in Human in Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.41 ng\/mL,\u003cbr\u003e\nRange:0.41 - 100 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman ASAH2 ELISA kit is an\u003cbr\u003e\nin vitro\u003cbr\u003e\nenzyme-linked immunosorbent assay for the quantitative measurement of human ASAH2 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human ASAH2 coated on a 96-well plate.\u003cbr\u003e\nThis ELISA kit shows no cross-reactivity with the following cytokines tested: human Adenosine Deaminase\/ADA, AIF, AKR1C4 , BCL-2, BID, Calreticulin, Calreticulin-2, CD314 (NKG2D), CD39L3\/ENTPD3, CD51, CD99-L2, CDC25B, Cerberus 1, CHST2, Cochlin, CRELD2, DC-SIGNR\/CD299, eNOS, ENPP2\/Autotaxin, FABP4, Fc epsilon RI, FGF R5, GALNT2, GALNT3, GIF, GPR111, beta-Glucuronidase\/GUSB, Inhibin A, LILRB4\/CD85k\/ILT3, Neuroglycan C\/CSPG5, NKp46\/NCR1, Neuronal Pentraxin R\/NPTXR, ROR2, SCCA2, Siglec-2\/CD22, SIRP alpha\/CD172a, SorCS1, Trypsin 1\/PRSS1, Trypsin 3\/PRSS3.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nASAH2 also known as N-acylsphingosine amidohydrolase 2 is an important enzyme responsible for the hydrolysis of ceramides into sphingosine and free fatty acids. It has a molecular mass of approximately 85 kDa. ASAH2 is expressed in many tissues with prominent expression observed in the small intestine and brain. Its activity influences cell membrane integrity and intercellular communication which plays an essential role in the normal functioning of cells.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nASAH2 regulates the levels of ceramide and sphingosine both of which are important lipid signaling molecules. This enzyme exists within cells as part of a complex that maintains lipid homeostasis impacting numerous biological processes through ceramide metabolism. The balance between ceramide and sphingosine signaling is important for cell survival proliferation and apoptosis highlighting the importance of ASAH2 in these cellular dynamics.\u003cbr\u003e\nPathways\u003cbr\u003e\nASAH2 involves itself in the sphingolipid metabolism pathway which is significant for signal transduction and cellular communication. ASAH2 works closely with proteins like sphingosine kinase which further metabolizes sphingosine into sphingosine-1-phosphate (S1P) a lipid mediator with diverse functions. This pathway plays essential roles in inflammation immune response and cell growth demonstrating the interconnected nature of ASAH2's activity with other critical proteins.\u003cbr\u003e\nASAH2 has associations with metabolic disorders and neurodegenerative diseases. Elevated ceramide levels due to ASAH2 dysfunction relate to disorders such as Gaucher disease characterized by abnormal lipid storage and may influence neurodegenerative conditions like Alzheimer's disease. The enzyme interacts with proteins like acid ceramidase where imbalances in these enzymes lead to disruptions in lipid metabolism and contribute to disease pathology making ASAH2 a potential target for therapeutic intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843784364201,"sku":"ab313510","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab313510","provider":"Iright","version":"1.0","type":"link"}