{"product_id":"abcam-ab314538","title":"Abcam, ab314538, Recombinant Dog CD20 protein - Active (His tag)","description":"\u003cp\u003eSize: 20µg \/ 100µg\u003cbr\u003e\nRecombinant Dog CD20 protein - Active (His tag) is a Dog Full Length protein, in the 1 to 297 aa range, expressed in Mammalian, \u0026lt; 1 EU\/µg endotoxin level, suitable for WB, FuncS.\u003cbr\u003e\nKey facts\u003cbr\u003e\nEndotoxin level:\u0026lt; 1 EU\/µg,\u003cbr\u003e\nExpression system:Mammalian,\u003cbr\u003e\nTags:His tag C-Terminus,\u003cbr\u003e\nApplications:FuncS, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,\u003cbr\u003e\nBiologically active:Yes,\u003cbr\u003e\nBiological activity:Measured by its binding ability in a functional ELISA. Immobilized AB314538 at 10 μg\/mL can bind an Anti-CD20 recombinant antibody, the EC50 is 147.3-199.0 ng\/mL.,\u003cbr\u003e\nAccession:Q3C2E2,\u003cbr\u003e\nAnimal free:Yes,\u003cbr\u003e\nCarrier free:No,\u003cbr\u003e\nSpecies:Dog,\u003cbr\u003e\nReconstitution:Reconstitute in water,\u003cbr\u003e\nStorage buffer:pH: 7.4 - 8Constituents: PBS, 6% Trehalose\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg\/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20degC\/-80degC. Our default final concentration of glycerol is 50%.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, -80°C, Appropriate long-term storage conditions--20°C, -80°C, Storage information-Avoid freeze \/ thaw cycle\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nCD20 also known as MS4A1 or L26 is a membrane-spanning 4-domains subfamily A member 1 protein. It is a non-glycosylated phosphoprotein with a molecular mass of approximately 33-37 kDa. Expressed extensively on the surface of B-cells CD20 plays an important role in B-cell activation and regulation. While CD20 is absent on early pro-B cells and plasma cells its presence increases as B-cells mature. Anti-CD20 antibodies such as 2H7 are commonly used in research and treatment to deplete B-cells due to the target's consistent expression in most stages of B-cell development.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nCD20 contributes significantly to calcium ion transport which is essential for the activation process of B-cells. Although CD20 does not belong to a larger protein complex its role centers around forming a calcium channel that allows a sustained influx of calcium into the B-cells. This influx is important for the signaling pathways that govern B-cell activation proliferation and differentiation impacting the immune response efficacy.\u003cbr\u003e\nPathways\u003cbr\u003e\nCD20 is closely involved in the B-cell receptor (BCR) signaling pathway and the Fc gamma R-mediated phagocytosis pathway. These pathways play vital roles in adaptive immunity and immune response modulation. CD20's interaction with proteins like PI3K during BCR signaling enhances receptor-mediated cellular signals subsequently influencing downstream effectors involved in cell growth and survival of B-cells.\u003cbr\u003e\nCD20 is strongly linked to certain blood cancers and autoimmune diseases including non-Hodgkin's lymphoma and rheumatoid arthritis. These conditions often exploit aberrant B-cell activity or count. CD20's expression on malignant B-cells in non-Hodgkin's lymphoma makes it an effective target for monoclonal antibody therapies such as rituximab an anti-CD20 antibody. Additionally CD20-targeted therapies can help deplete pathogenic B-cells in rheumatoid arthritis highlighting the protein's relevance in disease treatment.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843895152809,"sku":"ab314538","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab314538","provider":"Iright","version":"1.0","type":"link"}