{"product_id":"abcam-ab314605","title":"Abcam, ab314605, Monkey IL-12 p70 ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nMonkey IL-12 p70 ELISA Kit is a single-wash 90-min Simplestep used to quantify Monkey IL-12 p70 with a sensitivity of 35.887 pg\/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media,\u003cbr\u003e\nReacts with:Monkey,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 35.887 pg\/mL,\u003cbr\u003e\nRange:187.5 - 12000 pg\/mL,\u003cbr\u003e\nAssay time:1h 30m,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nMonkey IL-12 p70 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-12 p70 protein in Serum, EDTA Plasma, Hep Plasma, Cit Plasma, Cell culture media. Quantitate Monkey IL-12 p70 with 35.887 pg\/ml sensitivity. SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:\u003cbr\u003e\nSingle-wash protocol reduces assay time to 90 minutes or less\u003cbr\u003e\n-High sensitivity, specificity and reproducibility from superior antibodies\u003cbr\u003e\n-Fully validated in biological samples\u003cbr\u003e\n-96-wells plate breakable into 12 x 8 wells strips\u003cbr\u003e\nA 384-well SimpleStep ELISA® microplate (\u003cbr\u003e\nab203359\u003cbr\u003e\n) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nIL12 p70 also known as Interleukin-12 p70 plays a significant role in the immune response. It is a heterodimeric cytokine made up of two subunits p35 and p40 which together form the active p70. The molecular mass of the complete IL12 p70 molecule is approximately 70 kDa. It is primarily produced by activated dendritic cells and macrophages found mostly within the lymphoid tissues and at sites of inflammation.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nIL12 p70 functions to bridge innate and adaptive immunity. It induces the differentiation of naive T cells into Th1 cells an important process for the immune response. This cytokine is part of the IL12 family and functions as a complex with its receptor IL12R. It promotes the production of interferon-gamma (IFN-γ) from T and NK cells enhancing the cytotoxic activities that are important for protection against intracellular pathogens.\u003cbr\u003e\nPathways\u003cbr\u003e\nIL12 p70 is essential in the signaling pathways involved in cell-mediated immunity. It engages the JAK-STAT signaling pathway together with the IFN-γ pathway to activate immune responses against viral and bacterial infections. IL12 p70 interacts with proteins such as STAT4 which is key in transmitting its signal within the immune cells leading to the expression of immune response genes.\u003cbr\u003e\nIL12 p70 plays an important role in inflammatory diseases and autoimmune conditions. Elevated levels of IL12 p70 are associated with diseases like Crohn's disease where it contributes to chronic inflammation through increased IFN-γ production. It is also implicated in multiple sclerosis a result of its ability to promote Th1 differentiation and inflammation. In these conditions IL12 p70 is often connected with other cytokines like TNF-alpha which collectively exacerbate the inflammatory responses.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843640185001,"sku":"ab314605","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab314605","provider":"Iright","version":"1.0","type":"link"}