{"product_id":"abcam-ab314704","title":"Abcam, ab314704, Human Thimet Oligopeptidase ELISA Kit","description":"\u003cp\u003eSize: 1 x 96Tests\u003cbr\u003e\nHuman Thimet Oligopeptidase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Thimet Oligopeptidase in Human in Cell Culture Media, Biofluids samples.\u003cbr\u003e\nKey facts\u003cbr\u003e\nDetection method:Colorimetric,\u003cbr\u003e\nSample types:Cell culture supernatant, Plasma, Serum,\u003cbr\u003e\nReacts with:Human,\u003cbr\u003e\nAssay type:Sandwich (quantitative),\u003cbr\u003e\nSensitivity:= 0.9 ng\/mL,\u003cbr\u003e\nRange:0.35 - 200 ng\/mL,\u003cbr\u003e\nAssay Platform:Pre-coated microplate (12 x 8 well strips)\u003c\/p\u003e\n\n\u003cp\u003eProduct details:\u003cbr\u003e\nHuman Thimet Oligopeptidase ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Thimet Oligopeptidase in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Thimet Oligopeptidase coated on a 96-well plate.\u003c\/p\u003e\n\n\u003cp\u003eProperties and Storage Information:\u003cbr\u003e\nShipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C\u003c\/p\u003e\n\n\u003cp\u003eSupplementary Information:\u003cbr\u003e\nThis supplementary information is collated from multiple sources and compiled automatically.\u003cbr\u003e\nThimet Oligopeptidase also known as EC 3.4.24.15 or TOP functions as a zinc-dependent metallopeptidase. It breaks down peptides that are up to 20 amino acids long by cleaving peptide bonds. It has a molecular mass of approximately 78 kDa. This enzyme is expressed in various tissues prominently in the brain but also found in the kidneys lungs and testis reflecting its broad physiological roles. TOP primarily exists in the cytosol but can translocate to other cellular compartments under specific conditions.\u003cbr\u003e\nBiological function summary\u003cbr\u003e\nThimet Oligopeptidase plays a significant role in modulating neuropeptide levels in the nervous system. It does not operate as part of a known complex but instead acts independently in intracellular peptide catabolism. This activity helps regulate processes such as pain modulation and neuroendocrine signaling by modifying peptide-based signaling molecules. Furthermore TOP participates in the degradation of antigenic peptides implicating a role in immune system regulation.\u003cbr\u003e\nPathways\u003cbr\u003e\nThimet Oligopeptidase participates in neuropeptide and peptide hormone metabolism where it helps maintain homeostasis by modulating bioactive peptide concentrations. Within these pathways TOP interacts with other proteins such as neprilysin and angiotensin-converting enzyme creating a network that helps regulate cardiovascular and neural functions. This interaction highlights the enzyme's versatile capability to influence multiple signaling cascades.\u003cbr\u003e\nThimet Oligopeptidase connects strongly to neurodegenerative diseases such as Alzheimer's and Parkinson's. Research indicates that abnormal activity of TOP contributes to the accumulation of toxic peptide fragments like amyloid-beta in Alzheimer’s disease. This connection brings it into association with proteins like amyloid precursor protein (APP) and tau which are key in such neurodegenerative conditions. The enzyme's regulation of peptidase activity in these contexts highlights its potential as a therapeutic target for disease intervention.\u003c\/p\u003e","brand":"Abcam","offers":[{"title":"Default Title","offer_id":46843761983657,"sku":"ab314704","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/abcam-ab314704","provider":"Iright","version":"1.0","type":"link"}